Literature DB >> 26335826

The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR.

V N Danilevich1, A V Machulin, V V Sorokin, A L Mulyukin.   

Abstract

This work is devoted to studying the mechanisms of formation of DNA-containing microparticles (MPs) during PCR. It was found that pyrophosphate, a byproduct of DNA synthesis, and magnesium cations are required for their formation, as evidenced by the results of biochemical and electron microscopy studies.

Entities:  

Mesh：

Substances：

Year:  2015        PMID： 26335826     DOI： 10.1134/S1607672915040171

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN： 1607-6729            Impact factor:   0.788


  15 in total

1.  [New morphotypes of condensed DNA microparticles formed in PCR with KlenTaq- and Taq-polymerases and with plasmid DNAs as templates].

Authors:  V N Danilevich; E A Vasilenko; E V Pechnikova; E V Grishin
Journal:  Mikrobiologiia       Date:  2012 Jan-Feb

2.  Condensation of DNA by trivalent cations. 1. Effects of DNA length and topology on the size and shape of condensed particles.

Authors:  P G Arscott; A Z Li; V A Bloomfield
Journal:  Biopolymers       Date:  1990       Impact factor: 2.505

3.  [Amplification of the phage lambda DNA sequence by polymerase chain reaction using thermostable DNA polymerase].

Authors:  A I Glukhov; M E Trofimova; S A Gordeev; T V Grebennikova; S V Vinogradov; V I Kiselev; V M Kramarov
Journal:  Mol Biol (Mosk)       Date:  1991 Nov-Dec

4.  The structural peculiarities of condensed DNA micro- and nanoparticles formed in PCR.

Authors:  Vasily N Danilevich; Vladimir V Artemov; Steven S Smith; Radmir V Gainutdinov; Andrey L Mulyukin
Journal:  J Biomol Struct Dyn       Date:  2013-11-20

5.  RNAi-microsponges form through self-assembly of the organic and inorganic products of transcription.

Authors:  Kevin E Shopsowitz; Young Hoon Roh; Zhou J Deng; Stephen W Morton; Paula T Hammond
Journal:  Small       Date:  2014-04-24       Impact factor: 13.281

6.  [Microparticles from coupled DNA formed in the process of polymerase chain reaction].

Authors:  V N Danilevich; E S Barinova; E V Grishin
Journal:  Bioorg Khim       Date:  2009 Mar-Apr

7.  A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay.

Authors:  A A Baykov; O A Evtushenko; S M Avaeva
Journal:  Anal Biochem       Date:  1988-06       Impact factor: 3.365

8.  Differences in DNA condensation and release by lysine and arginine homopeptides govern their DNA delivery efficiencies.

Authors:  Anita Mann; Garima Thakur; Vasundhara Shukla; Anand Kamal Singh; Richa Khanduri; Rangeetha Naik; Yang Jiang; Namita Kalra; B S Dwarakanath; Ulo Langel; Munia Ganguli
Journal:  Mol Pharm       Date:  2011-08-05       Impact factor: 4.939

9.  Formation of DNA nanoparticles in the presence of novel polyamine analogues: a laser light scattering and atomic force microscopic study.

Authors:  Veena Vijayanathan; Thresia Thomas; Thomas Antony; Akira Shirahata; T J Thomas
Journal:  Nucleic Acids Res       Date:  2004-01-02       Impact factor: 16.971

10.  Characterization of DNA condensates induced by poly(ethylene oxide) and polylysine.

Authors:  U K Laemmli
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205
View more
  1 in total

1.  Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System.

Authors:  Daniel Hürtgen; Judita Mascarenhas; Michael Heymann; Seán M Murray; Petra Schwille; Victor Sourjik
Journal:  Chembiochem       Date:  2019-08-28       Impact factor: 3.164
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.