Shu-Zhong Chen1, Jing Yuan2, Min Deng1, Juan Wei3, Jinyuan Zhou4,5, Yì-Xiáng J Wáng6. 1. Department of Imaging and Interventional Radiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong, SAR. 2. Medical Physics and Research Department, Hong Kong Sanatorium & Hospital, Happy Valley, Hong Kong, SAR. 3. Philips Healthcare Asia, Shanghai, China. 4. Department of Radiology, Johns Hopkins University, Baltimore, MD, USA. 5. F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, USA. 6. Department of Imaging and Interventional Radiology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong, SAR. yixiang_wang@cuhk.edu.hk.
Abstract
PURPOSE: To evaluate Chemical Exchange Saturation Transfer (CEST) MRI for liver imaging at 3.0-T. MATERIALS AND METHODS: Images were acquired at offsets (n = 41, increment = 0.25 ppm) from -5 to 5 ppm using a TSE sequence with a continuous rectangular saturation pulse. Amide proton transfer-weighted (APTw) and GlycoCEST signals were quantified as the asymmetric magnetization transfer ratio (MTRasym) at 3.5 ppm and the total MTRasym integrated from 0.5 to 1.5 ppm, respectively, from the corrected Z-spectrum. Reproducibility was assessed for rats and humans. Eight rats were devoid of chow for 24 hours and scanned before and after fasting. Eleven rats were scanned before and after one-time CCl4 intoxication. RESULTS: For reproducibility, rat liver APTw and GlycoCEST measurements had 95 % limits of agreement of -1.49 % to 1.28 % and -0.317 % to 0.345 %. Human liver APTw and GlycoCEST measurements had 95 % limits of agreement of -0.842 % to 0.899 % and -0.344 % to 0.164 %. After 24 hours, fasting rat liver APTw and GlycoCEST signals decreased from 2.38 ± 0.86 % to 0.67 ± 1.12 % and from 0.34 ± 0.26 % to -0.18 ± 0.37 % respectively (p < 0.05). After CCl4 intoxication rat liver APTw and GlycoCEST signals decreased from 2.46 ± 0.48 % to 1.10 ± 0.77 %, and from 0.34 ± 0.23 % to -0.16 ± 0.51 % respectively (p < 0.05). CONCLUSION: CEST liver imaging at 3.0-T showed high sensitivity for fasting as well as CCl4 intoxication. KEY POINTS: • CEST MRI of in-vivo liver was demonstrated at clinical 3 T field strength. • After 24-hour fasting, rat liver APTw and GlycoCEST signals decreased significantly. • After CCl4 intoxication both rat liver APTw and GlycoCEST signals decreased significantly. • Good scan-rescan reproducibility of liver CEST MRI was shown in healthy volunteers.
PURPOSE: To evaluate Chemical Exchange Saturation Transfer (CEST) MRI for liver imaging at 3.0-T. MATERIALS AND METHODS: Images were acquired at offsets (n = 41, increment = 0.25 ppm) from -5 to 5 ppm using a TSE sequence with a continuous rectangular saturation pulse. Amide proton transfer-weighted (APTw) and GlycoCEST signals were quantified as the asymmetric magnetization transfer ratio (MTRasym) at 3.5 ppm and the total MTRasym integrated from 0.5 to 1.5 ppm, respectively, from the corrected Z-spectrum. Reproducibility was assessed for rats and humans. Eight rats were devoid of chow for 24 hours and scanned before and after fasting. Eleven rats were scanned before and after one-time CCl4 intoxication. RESULTS: For reproducibility, rat liver APTw and GlycoCEST measurements had 95 % limits of agreement of -1.49 % to 1.28 % and -0.317 % to 0.345 %. Human liver APTw and GlycoCEST measurements had 95 % limits of agreement of -0.842 % to 0.899 % and -0.344 % to 0.164 %. After 24 hours, fasting rat liver APTw and GlycoCEST signals decreased from 2.38 ± 0.86 % to 0.67 ± 1.12 % and from 0.34 ± 0.26 % to -0.18 ± 0.37 % respectively (p < 0.05). After CCl4 intoxication rat liver APTw and GlycoCEST signals decreased from 2.46 ± 0.48 % to 1.10 ± 0.77 %, and from 0.34 ± 0.23 % to -0.16 ± 0.51 % respectively (p < 0.05). CONCLUSION: CEST liver imaging at 3.0-T showed high sensitivity for fasting as well as CCl4 intoxication. KEY POINTS: • CEST MRI of in-vivo liver was demonstrated at clinical 3 T field strength. • After 24-hour fasting, rat liver APTw and GlycoCEST signals decreased significantly. • After CCl4 intoxication both rat liver APTw and GlycoCEST signals decreased significantly. • Good scan-rescan reproducibility of liver CEST MRI was shown in healthy volunteers.
Entities:
Keywords:
Amide proton transfer (APT); Chemical exchange saturation transfer (CEST); Glycogen; Liver; Magnetic resonance imaging
Authors: Phillip Zhe Sun; Enfeng Wang; Jerry S Cheung; Xiaoan Zhang; Thomas Benner; A Gregory Sorensen Journal: Magn Reson Med Date: 2011-03-24 Impact factor: 4.668
Authors: Kannie W Y Chan; Michael T McMahon; Yoshinori Kato; Guanshu Liu; Jeff W M Bulte; Zaver M Bhujwalla; Dmitri Artemov; Peter C M van Zijl Journal: Magn Reson Med Date: 2012-10-16 Impact factor: 4.668
Authors: April M Chow; Darwin S Gao; Shu Juan Fan; Zhongwei Qiao; Frank Y Lee; Jian Yang; Kwan Man; Ed X Wu Journal: J Magn Reson Imaging Date: 2012-02-14 Impact factor: 4.813
Authors: Craig K Jones; Alan Huang; Jiadi Xu; Richard A E Edden; Michael Schär; Jun Hua; Nikita Oskolkov; Domenico Zacà; Jinyuan Zhou; Michael T McMahon; Jay J Pillai; Peter C M van Zijl Journal: Neuroimage Date: 2013-04-06 Impact factor: 6.556
Authors: Huanling Liu; Anna Jablonska; Yuguo Li; Suyi Cao; Dexiang Liu; Hanwei Chen; Peter Cm Van Zijl; Jeff W M Bulte; Miroslaw Janowski; Piotr Walczak; Guanshu Liu Journal: Theranostics Date: 2016-06-18 Impact factor: 11.556