Literature DB >> 26324265

Functions of a GyrBA fusion protein and its interaction with QnrB and quinolones.

Chunhui Chen1, Regis Villet2, George A Jacoby3, David C Hooper4.   

Abstract

In order to study the interactions between Escherichia coli DNA gyrase and the gyrase interacting protein QnrB in vivo, we constructed a gyrB-gyrA fusion and validated its ability to correct the temperature-sensitive growth of gyrA and gyrB mutants. Like wild-type gyrA, the gyrB-gyrA fusion complemented a quinolone-resistant gyrA mutant to increase susceptibility. It functioned as an active type II topoisomerase, catalyzed negative supercoiling of DNA, was inhibited by quinolone, and was protected by QnrB.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 26324265      PMCID： PMC4604398          DOI： 10.1128/AAC.01845-15

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  17 in total

1.  DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

2.  A GyrB-GyrA fusion protein expressed in yeast cells is able to remove DNA supercoils but cannot substitute eukaryotic topoisomerase II.

Authors:  Sonia Trigueros; Joaquim Roca
Journal:  Genes Cells       Date:  2002-03       Impact factor: 1.891

3.  Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling.

Authors:  R Menzel; M Gellert
Journal:  Cell       Date:  1983-08       Impact factor: 41.582

4.  DNA gyrase-mediated wrapping of the DNA strand is required for the replication fork arrest by the DNA gyrase-quinolone-DNA ternary complex.

Authors:  H Hiasa; M E Shea
Journal:  J Biol Chem       Date:  2000-11-03       Impact factor: 5.157

5.  Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli.

Authors:  H Yoshida; M Bogaki; M Nakamura; S Nakamura
Journal:  Antimicrob Agents Chemother       Date:  1990-06       Impact factor: 5.191

6.  Mechanism of plasmid-mediated quinolone resistance.

Authors:  John H Tran; George A Jacoby
Journal:  Proc Natl Acad Sci U S A       Date:  2002-04-09       Impact factor: 11.205

7.  Detection of extended-spectrum beta-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli.

Authors:  G A Jacoby; P Han
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

8.  Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity.

Authors:  N P Higgins; C L Peebles; A Sugino; N R Cozzarelli
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

Review 9.  Fluoroquinolones: structure and target sites.

Authors:  P G Higgins; A C Fluit; F J Schmitz
Journal:  Curr Drug Targets       Date:  2003-02       Impact factor: 3.465

10.  DNA topoisomerase VIII: a novel subfamily of type IIB topoisomerases encoded by free or integrated plasmids in Archaea and Bacteria.

Authors:  Danièle Gadelle; Mart Krupovic; Kasie Raymann; Claudine Mayer; Patrick Forterre
Journal:  Nucleic Acids Res       Date:  2014-07-02       Impact factor: 16.971
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