Literature DB >> 26323263

Development of an agroinoculation system for full-length and GFP-tagged cDNA clones of cucumber green mottle mosaic virus.

Hongying Zheng1, Caili Xiao1,2, Kelei Han1,2, Jiejun Peng1, Lin Lin1, Yuwen Lu1, Li Xie1, Xiaohua Wu3, Pei Xu3, Guojing Li3, Jianping Chen4, Fei Yan5.   

Abstract

The complete 6243-nucleotide sequence of a cucumber green mottle mosaic virus (CGMMV) isolate from bottle gourd in Zhejiang province, China, was determined. A full-length cDNA clone of this isolate was constructed by inserting the cDNA between the 35S promoter and the ribozyme in the binary plasmid pCB301-CH. A suspension of an Agrobacterium tumefaciens EHA105 clone carrying this construct was highly infectious in Nicotiana benthamiana and bottle gourd. Another infectious clone containing the green fluorescence protein (GFP) reporter gene was also successfully constructed. This study is the first report of the efficient use of agroinoculation for generating CGMMV infections.

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Year:  2015        PMID： 26323263     DOI： 10.1007/s00705-015-2584-y

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  8 in total

1.  Complete nucleotide sequences and construction of full-length infectious cDNA clones of cucumber green mottle mosaic virus (CGMMV) in a versatile newly developed binary vector including both 35S and T7 promoters.

Authors:  Chan-Hwan Park; Hye-Kyoung Ju; Jae-Yeong Han; Jong-Seo Park; Ik-Hyun Kim; Eun-Young Seo; Jung-Kyu Kim; John Hammond; Hyoun-Sub Lim
Journal:  Virus Genes       Date:  2016-12-02       Impact factor: 2.332

2.  Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR.

Authors:  Yin Xinying; Li Xin; Yang Lili; Zheng Qiuyue; Piao Yongzhe; Cao Jijuan
Journal:  Plant Methods       Date:  2022-05-26       Impact factor: 5.827

3.  Single amino acid substitutions in the coat protein and RNA-dependent RNA polymerase alleviated the virulence of Cucumber green mottle mosaic virus and conferred cross protection against severe infection.

Authors:  Jin Liu; Xiang-Dong Li; Shuai Xu
Journal:  Virus Genes       Date:  2020-01-01       Impact factor: 2.332

4.  Systemic Propagation of a Fluorescent Infectious Clone of a Polerovirus Following Inoculation by Agrobacteria and Aphids.

Authors:  Sylvaine Boissinot; Elodie Pichon; Céline Sorin; Céline Piccini; Danièle Scheidecker; Véronique Ziegler-Graff; Véronique Brault
Journal:  Viruses       Date:  2017-06-29       Impact factor: 5.048

5.  Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation.

Authors:  Decai Tuo; Lanlan Fu; Wentao Shen; Xiaoying Li; Peng Zhou; Pu Yan
Journal:  Virology       Date:  2017-07-14       Impact factor: 3.616

6.  A cucumber green mottle mosaic virus vector for virus-induced gene silencing in cucurbit plants.

Authors:  Mei Liu; Zhiling Liang; Miguel A Aranda; Ni Hong; Liming Liu; Baoshan Kang; Qinsheng Gu
Journal:  Plant Methods       Date:  2020-02-03       Impact factor: 4.993

7.  Genome-wide identification of new reference genes for RT-qPCR normalization in CGMMV-infected Lagenaria siceraria.

Authors:  Chenhua Zhang; Hongying Zheng; Xinyang Wu; Heng Xu; Kelei Han; Jiejun Peng; Yuwen Lu; Lin Lin; Pei Xu; Xiaohua Wu; Guojing Li; Jianping Chen; Fei Yan
Journal:  PeerJ       Date:  2018-10-12       Impact factor: 2.984

8.  A single amino acid in coat protein of Pepper mild mottle virus determines its subcellular localization and the chlorosis symptom on leaves of pepper.

Authors:  Kelei Han; Hongying Zheng; Mengfei Ji; Weijun Cui; Shuzhen Hu; Jiejun Peng; Jinping Zhao; Yuwen Lu; Lin Lin; Yong Liu; Jianping Chen; Fei Yan
Journal:  J Gen Virol       Date:  2020-03-09       Impact factor: 3.891
  8 in total

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