Andrea Iorio1, Marina Spinelli1, Renato Polimanti2, Federica Lorenzi3, Herbert Valensise4, Dario Manfellotto3, Maria Fuciarelli5. 1. Department of Biology, University of Rome "Tor Vergata", Rome, Italy. 2. Department of Psychiatry, Yale University School of Medicine, West Haven, CT, United States. 3. Clinical Pathophysiology Center, AFaR Division, Fatebenefratelli Foundation, "San Giovanni Calibita" Fatebenefratelli Hospital, Isola Tiberina, Rome, Italy. 4. Department of Obstetrics and Gynecology, University of Rome "Tor Vergata", Fatebenefratelli Hospital, Isola Tiberina, Rome, Italy. 5. Department of Biology, University of Rome "Tor Vergata", Rome, Italy. Electronic address: fuciarelli@uniroma2.it.
Abstract
OBJECTIVE(S): Glutathione S-transferases (GSTs) are the main phase II enzymes involved in the cellular detoxification. Through phase I and phase II detoxification reactions, the cell is able to detoxify endogenous and exogenous toxic compounds. In this study, we focused our attention on the GSTA1*-69C/T gene polymorphism (rs3957357) in order to explore its involvement in the genetic predisposition to gestational hypertension (GH). STUDY DESIGN: The case-control population consists of 195 subjects. The genotyping of the GSTA1*-69C/T was performed by using an RFLP-PCR technique. We calculated odds ratios (ORs), adjusted for the confounding variables, to estimate the association between GSTA1 and GH. RESULTS: Significant allelic differences in GSTA1*-69C/T are present between GH women and pregnant women without cardiovascular complications (p<0.05). Specifically, we observed that the dominant genetic model best explains the observed genetic association, according to the Akaike information criterion and the Bayesian information criterion. CONCLUSION(S): Our study highlighted a significant association between the GSTA1 gene and the risk of GH in Italian patients. In particular, the -69C/T variant was significantly associated with disease risk. Since previous studies indicated that this GSTA1 polymorphism is associated with different pregnancy-related conditions, our finding supports the notion that GSTA1 may play a key role during pregnancy.
OBJECTIVE(S): Glutathione S-transferases (GSTs) are the main phase II enzymes involved in the cellular detoxification. Through phase I and phase II detoxification reactions, the cell is able to detoxify endogenous and exogenous toxic compounds. In this study, we focused our attention on the GSTA1*-69C/T gene polymorphism (rs3957357) in order to explore its involvement in the genetic predisposition to gestational hypertension (GH). STUDY DESIGN: The case-control population consists of 195 subjects. The genotyping of the GSTA1*-69C/T was performed by using an RFLP-PCR technique. We calculated odds ratios (ORs), adjusted for the confounding variables, to estimate the association between GSTA1 and GH. RESULTS: Significant allelic differences in GSTA1*-69C/T are present between GH women and pregnant women without cardiovascular complications (p<0.05). Specifically, we observed that the dominant genetic model best explains the observed genetic association, according to the Akaike information criterion and the Bayesian information criterion. CONCLUSION(S): Our study highlighted a significant association between the GSTA1 gene and the risk of GH in Italian patients. In particular, the -69C/T variant was significantly associated with disease risk. Since previous studies indicated that this GSTA1 polymorphism is associated with different pregnancy-related conditions, our finding supports the notion that GSTA1 may play a key role during pregnancy.