Maria Tereza P Albuquerque1,2, Joshua D Evans1, Richard L Gregory1, Marcia C Valera2, Marco C Bottino3,4,5. 1. Department of Biomedical and Applied Sciences, Indiana University School of Dentistry (IUSD), 1121 W. Michigan Street, Indianapolis, IN, 46202, USA. 2. Graduate Program in Restorative Dentistry (Endodontics), São José dos Campos Dental School, Universidade Estadual Paulista, São José dos Campos, São Paulo, 12245-000, Brazil. 3. Department of Biomedical and Applied Sciences, Indiana University School of Dentistry (IUSD), 1121 W. Michigan Street, Indianapolis, IN, 46202, USA. mbottino@iu.edu. 4. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, 46202, USA. mbottino@iu.edu. 5. Department of Biomedical Engineering, Purdue School of Engineering and Technology, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202, USA. mbottino@iu.edu.
Abstract
OBJECTIVES: This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)-mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. MATERIALS AND METHODS: Dentin specimens (4 × 4 × 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP [25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5% level. RESULTS: Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log10 CFU/mL) and the negative control (5.9 log10 CFU/mL). CONCLUSIONS: The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. CLINICAL RELEVANCE: Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.
OBJECTIVES: This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)-mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm. MATERIALS AND METHODS: Dentin specimens (4 × 4 × 1 mm(3)) were prepared from humancanines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP [25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5% level. RESULTS: Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log10 CFU/mL) and the negative control (5.9 log10 CFU/mL). CONCLUSIONS: The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm. CLINICAL RELEVANCE: Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.
Authors: Juliana Yuri Nagata; Brenda Paula Figueiredo de Almeida Gomes; Thiago Farias Rocha Lima; Lia Saori Murakami; Danielle Elaine de Faria; Gabriel Rocha Campos; Francisco José de Souza-Filho; Adriana de Jesus Soares Journal: J Endod Date: 2014-03-06 Impact factor: 4.171
Authors: Juliana Y Nagata; Adriana J Soares; Francisco J Souza-Filho; Alexandre A Zaia; Caio C R Ferraz; José F A Almeida; Brenda P F A Gomes Journal: J Endod Date: 2014-04-29 Impact factor: 4.171
Authors: David E Martin; Jose Flavio A De Almeida; Michael A Henry; Zin Z Khaing; Christine E Schmidt; Fabricio B Teixeira; Anibal Diogenes Journal: J Endod Date: 2013-10-25 Impact factor: 4.171
Authors: Ronald Ordinola-Zapata; Clovis M Bramante; Paloma Gagliardi Minotti; Bruno Cavalini Cavenago; Roberto Brandão Garcia; Norberti Bernardineli; David E Jaramillo; Marco A Hungaro Duarte Journal: J Endod Date: 2012-11-10 Impact factor: 4.171
Authors: Ángel Serrano-Aroca; Alba Cano-Vicent; Roser Sabater I Serra; Mohamed El-Tanani; AlaaAA Aljabali; Murtaza M Tambuwala; Yogendra Kumar Mishra Journal: Mater Today Bio Date: 2022-08-30
Authors: Divya Pankajakshan; Maria T P Albuquerque; Joshua D Evans; Malgorzata M Kamocka; Richard L Gregory; Marco C Bottino Journal: J Endod Date: 2016-10 Impact factor: 4.171
Authors: Juliana S Ribeiro; Eliseu A Münchow; Ester A Ferreira Bordini; Wellington Luiz de Oliveira da Rosa; Marco C Bottino Journal: J Endod Date: 2020-09 Impact factor: 4.171
Authors: Marco C Bottino; Maria T P Albuquerque; Asma Azabi; Eliseu A Münchow; Kenneth J Spolnik; Jacques E Nör; Paul C Edwards Journal: J Biomed Mater Res B Appl Biomater Date: 2018-10-03 Impact factor: 3.368
Authors: Manuel Toledano-Osorio; Jegdish P Babu; Raquel Osorio; Antonio L Medina-Castillo; Franklin García-Godoy; Manuel Toledano Journal: Materials (Basel) Date: 2018-06-14 Impact factor: 3.623
Authors: Juliana S Ribeiro; Eliseu A Münchow; Ester A F Bordini; Nathalie S Rodrigues; Nileshkumar Dubey; Hajime Sasaki; John C Fenno; Steven Schwendeman; Marco C Bottino Journal: Int J Mol Sci Date: 2022-01-16 Impact factor: 5.923