Literature DB >> 26318720

Derivation of LIF-independent mouse iPS cells with modified Oct4.

Hiroyuki Hirai1, Meri Firpo2, Nobuaki Kikyo3.   

Abstract

It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.
Copyright © 2015. Published by Elsevier B.V.

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Year:  2015        PMID： 26318720      PMCID： PMC4600661          DOI： 10.1016/j.scr.2015.08.005

Source DB:  PubMed          Journal:  Stem Cell Res        ISSN： 1873-5061            Impact factor:   2.020


  5 in total

1.  Plat-E: an efficient and stable system for transient packaging of retroviruses.

Authors:  S Morita; T Kojima; T Kitamura
Journal:  Gene Ther       Date:  2000-06       Impact factor: 5.250

Review 2.  Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics.

Authors:  Toshio Kitamura; Yuko Koshino; Fumi Shibata; Toshihiko Oki; Hideaki Nakajima; Tetsuya Nosaka; Hidetoshi Kumagai
Journal:  Exp Hematol       Date:  2003-11       Impact factor: 3.084

3.  Radical acceleration of nuclear reprogramming by chromatin remodeling with the transactivation domain of MyoD.

Authors:  Hiroyuki Hirai; Tetsuya Tani; Nobuko Katoku-Kikyo; Steven Kellner; Peter Karian; Meri Firpo; Nobuaki Kikyo
Journal:  Stem Cells       Date:  2011-09       Impact factor: 6.277

4.  Oct4 expression is not required for mouse somatic stem cell self-renewal.

Authors:  Christopher J Lengner; Fernando D Camargo; Konrad Hochedlinger; G Grant Welstead; Samir Zaidi; Sumita Gokhale; Hans R Scholer; Alexey Tomilin; Rudolf Jaenisch
Journal:  Cell Stem Cell       Date:  2007-10-11       Impact factor: 24.633

5.  A drug-inducible system for direct reprogramming of human somatic cells to pluripotency.

Authors:  Dirk Hockemeyer; Frank Soldner; Elizabeth G Cook; Qing Gao; Maisam Mitalipova; Rudolf Jaenisch
Journal:  Cell Stem Cell       Date:  2008-09-11       Impact factor: 24.633
  5 in total

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