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Literature DB >> 26315438

DNA RECOMBINATION. Base triplet stepping by the Rad51/RecA family of recombinases.

Ja Yil Lee¹, Tsuyoshi Terakawa², Zhi Qi¹, Justin B Steinfeld¹, Sy Redding³, YoungHo Kwon⁴, William A Gaines⁴, Weixing Zhao⁴, Patrick Sung⁴, Eric C Greene⁵.

Abstract

DNA strand exchange plays a central role in genetic recombination across all kingdoms of life, but the physical basis for these reactions remains poorly defined. Using single-molecule imaging, we found that bacterial RecA and eukaryotic Rad51 and Dmc1 all stabilize strand exchange intermediates in precise three-nucleotide steps. Each step coincides with an energetic signature (0.3 kBT) that is conserved from bacteria to humans. Triplet recognition is strictly dependent on correct Watson-Crick pairing. Rad51, RecA, and Dmc1 can all step over mismatches, but only Dmc1 can stabilize mismatched triplets. This finding provides insight into why eukaryotes have evolved a meiosis-specific recombinase. We propose that canonical Watson-Crick base triplets serve as the fundamental unit of pairing interactions during DNA recombination.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2015 PMID： 26315438 PMCID： PMC4580133 DOI： 10.1126/science.aab2666

Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728

19 in total

1. The importance of repairing stalled replication forks.

Authors: M M Cox; M F Goodman; K N Kreuzer; D J Sherratt; S J Sandler; K J Marians
Journal: Nature Date: 2000-03-02 Impact factor: 49.962

2. The role of the mismatch repair machinery in regulating mitotic and meiotic recombination between diverged sequences in yeast.

Authors: W Chen; S Jinks-Robertson
Journal: Genetics Date: 1999-04 Impact factor: 4.562

Review 3. Recombination and the nature of bacterial speciation.

Authors: Christophe Fraser; William P Hanage; Brian G Spratt
Journal: Science Date: 2007-01-26 Impact factor: 47.728

4. DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.

Authors: D K Bishop; D Park; L Xu; N Kleckner
Journal: Cell Date: 1992-05-01 Impact factor: 41.582

10. ATP hydrolysis provides functions that promote rejection of pairings between different copies of long repeated sequences.

Authors: Claudia Danilowicz; Laura Hermans; Vincent Coljee; Chantal Prévost; Mara Prentiss
Journal: Nucleic Acids Res Date: 2017-08-21 Impact factor: 16.971

DNA RECOMBINATION. Base triplet stepping by the Rad51/RecA family of recombinases.

1. The importance of repairing stalled replication forks.

2. The role of the mismatch repair machinery in regulating mitotic and meiotic recombination between diverged sequences in yeast.

Review 3. Recombination and the nature of bacterial speciation.

4. DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.

5. DNA sequence alignment by microhomology sampling during homologous recombination.

Review 6. Clarifying the mechanics of DNA strand exchange in meiotic recombination.

7. The Saccharomyces cerevisiae Msh2 mismatch repair protein localizes to recombination intermediates in vivo.

8. Mismatch correction acts as a barrier to homeologous recombination in Saccharomyces cerevisiae.

Review 9. DNA strand exchange proteins: a biochemical and physical comparison.

10. Base-stacking and base-pairing contributions into thermal stability of the DNA double helix.

1. Tolerance of DNA Mismatches in Dmc1 Recombinase-mediated DNA Strand Exchange.

2. Rad51-mediated double-strand break repair and mismatch correction of divergent substrates.

3. On the influence of protein-DNA register during homologous recombination.

4. Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis.

5. Single-Molecule Methods for Nucleotide Excision Repair: Building a System to Watch Repair in Real Time.

Review 6. The biochemistry of early meiotic recombination intermediates.

Review 7. A time for promiscuity in a eukaryotic recombinase.

Review 8. Biochemical attributes of mitotic and meiotic presynaptic complexes.

Review 9. RAD51 Gene Family Structure and Function.

10. ATP hydrolysis provides functions that promote rejection of pairings between different copies of long repeated sequences.