Andrew R Thoreson1, Ryo Hiwatari1, Kai-Nan An1, Peter C Amadio1, Chunfeng Zhao2. 1. Division of Orthopedic Research, Mayo Clinic, Biomechanics Laboratory, Rochester, MN. 2. Division of Orthopedic Research, Mayo Clinic, Biomechanics Laboratory, Rochester, MN. Electronic address: zhao.chunfeng@mayo.edu.
Abstract
PURPOSE: To determine if impregnating a suture with a cross-linking agent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), improved suture pull-out strength and cell viability. METHODS: Canine flexor digitorum profundus tendons were cut in canine zone D, and a single suture loop was placed in each end, with sutures soaked in either saline or an EDC solution with a concentration of 1%, 10%, or 50%. Suture pull-out strength, stiffness, and elongation to failure was determined by pulling the loop until failure. Cytotoxicity of the EDC treatment was evaluated by suspending treated sutures over cultured tenocytes. RESULTS: Mechanical properties for the EDC-treated side were improved over controls when treated with the 10% and 50% EDC solutions. The ratio of dead to live cells was significantly increased at all distances from the suture for the 50% EDC-treated group. CONCLUSIONS: Suture treated with a 10% EDC solution provided the best combination of mechanical reinforcement and limited toxicity. CLINICAL RELEVANCE: Sutures so treated may improve the ability of a tendon repair to sustain early mobilization.
PURPOSE: To determine if impregnating a suture with a cross-linking agent, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), improved suture pull-out strength and cell viability. METHODS:Canine flexor digitorum profundus tendons were cut in canine zone D, and a single suture loop was placed in each end, with sutures soaked in either saline or an EDC solution with a concentration of 1%, 10%, or 50%. Suture pull-out strength, stiffness, and elongation to failure was determined by pulling the loop until failure. Cytotoxicity of the EDC treatment was evaluated by suspending treated sutures over cultured tenocytes. RESULTS: Mechanical properties for the EDC-treated side were improved over controls when treated with the 10% and 50% EDC solutions. The ratio of dead to live cells was significantly increased at all distances from the suture for the 50% EDC-treated group. CONCLUSIONS: Suture treated with a 10% EDC solution provided the best combination of mechanical reinforcement and limited toxicity. CLINICAL RELEVANCE: Sutures so treated may improve the ability of a tendon repair to sustain early mobilization.
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