R Fekrazad1, M Sadeghi Ghuchani2, M B Eslaminejad3, L Taghiyar4, K A M Kalhori5, M S Pedram6, A M Shayan7, N Aghdami8, H Abrahamse9. 1. Dental Department, AJA University of Medical Sciences, Tehran, Iran. Electronic address: rezafekrazad@gmail.com. 2. Gorgan Faculty of Dentistry, Golestan University of Medical Sciences, Golestan, Iran. Electronic address: moss.sadeghi@gmail.com. 3. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Electronic address: eslami@royaninstitute.org. 4. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Electronic address: leila_taghiyar@yahoo.com. 5. Iranian Medical Laser Association, Tehran, Iran. Electronic address: kathykalhor@yahoo.com. 6. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Iran. Electronic address: mpedram@ut.ac.ir. 7. Tabriz Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: sh_arman_ima@yahoo.com. 8. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Electronic address: Nasser.Aghdami@ki.se. 9. Laser Research Center, Faculty of Health Sciences, University of Johannesburg, South Africa. Electronic address: habrahamse@uj.ac.za.
Abstract
OBJECTIVE: This study evaluated the effect of Low Level Laser Therapy (LLLT) and Mesenchymal Stem Cells (MSCs) on bone regeneration. BACKGROUND DATA: Although several studies evaluated the effects of MSCs and LLLT, there is little information available regarding in vivo application of LLLT in conjunction with MSCs. METHODS: Forty-eight circular bone defects (6mm in diameter) were prepared in the calvaria of 12 New-Zealand white rabbits. The defects of each animal were randomly assigned to 4 groups: (C) no treatment; (L) applying LLLT; (SC) filled with MSCs; (SCL) application of both MSCs and LLLT. LLL was applied on alternate days at wavelength of 810 nm, power density of 0.2 W/cm(2) and a fluency of 4 J/cm(2) using a Gallium-Aluminum-Arsenide (GaAlAs) diode laser. The animals were sacrificed after 3 weeks and then histological samples were evaluated to determine the amount of new bone formation and the remaining scaffold and inflammation. RESULTS: The histological evaluation showed a statistically significant increase in new bone formation of LLLT group relative to the control and the other two experimental groups (p<0.05). There was no significant difference in bone formation of the control group compared to experimental groups filled with MSCs. Laser irradiation had no significant effect on resorption of the scaffold material. In addition, inflammation was significantly reduced in LLLT group compared to the control defects and the other two experimental groups. CONCLUSION: Low level laser therapy could be effective in bone regeneration but there is no evidence of a synergistic effect when applied in conjunction with MSCs.
OBJECTIVE: This study evaluated the effect of Low Level Laser Therapy (LLLT) and Mesenchymal Stem Cells (MSCs) on bone regeneration. BACKGROUND DATA: Although several studies evaluated the effects of MSCs and LLLT, there is little information available regarding in vivo application of LLLT in conjunction with MSCs. METHODS: Forty-eight circular bone defects (6mm in diameter) were prepared in the calvaria of 12 New-Zealand white rabbits. The defects of each animal were randomly assigned to 4 groups: (C) no treatment; (L) applying LLLT; (SC) filled with MSCs; (SCL) application of both MSCs and LLLT. LLL was applied on alternate days at wavelength of 810 nm, power density of 0.2 W/cm(2) and a fluency of 4 J/cm(2) using a Gallium-Aluminum-Arsenide (GaAlAs) diode laser. The animals were sacrificed after 3 weeks and then histological samples were evaluated to determine the amount of new bone formation and the remaining scaffold and inflammation. RESULTS: The histological evaluation showed a statistically significant increase in new bone formation of LLLT group relative to the control and the other two experimental groups (p<0.05). There was no significant difference in bone formation of the control group compared to experimental groups filled with MSCs. Laser irradiation had no significant effect on resorption of the scaffold material. In addition, inflammation was significantly reduced in LLLT group compared to the control defects and the other two experimental groups. CONCLUSION: Low level laser therapy could be effective in bone regeneration but there is no evidence of a synergistic effect when applied in conjunction with MSCs.
Authors: Ruxandra Elena Luca; Carmen Darinca Todea; Virgil-Florin Duma; Adrian Bradu; Adrian Gh Podoleanu Journal: Quant Imaging Med Surg Date: 2019-05
Authors: Polina Y Bikmulina; Nastasia V Kosheleva; Anastasia I Shpichka; Yuri M Efremov; Vladimir I Yusupov; Peter S Timashev; Yury A Rochev Journal: J Biomed Opt Date: 2020-04 Impact factor: 3.170