Literature DB >> 26280528

Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase.

Thomas Wiegand1, Carole Gardiennet2, Francesco Ravotti1, Alexandre Bazin2, Britta Kunert2, Denis Lacabanne2, Riccardo Cadalbert1, Peter Güntert1,3, Laurent Terradot4, Anja Böckmann5, Beat H Meier6.   

Abstract

We present solid-state NMR assignments of the N-terminal domain of the DnaB helicase from Helicobacter pylori (153 residues) in its microcrystalline form. We use a sequential resonance assignment strategy based on three-dimensional NMR experiments. The resonance assignments obtained are compared with automated resonance assignments computed with the ssFLYA algorithm. An analysis of the (13)C secondary chemical shifts determines the position of the secondary structure elements in this α-helical protein.

Entities:  

Keywords:  Assignments; HpDnaB; Secondary chemical shifts; Solid-state NMR; ssFLYA

Mesh：

Substances：

Year:  2015        PMID： 26280528     DOI： 10.1007/s12104-015-9629-8

Source DB:  PubMed          Journal:  Biomol NMR Assign        ISSN： 1874-270X            Impact factor:   0.746


  8 in total

1.  Segmental isotope labelling and solid-state NMR of a 12 × 59 kDa motor protein: identification of structural variability.

Authors:  Thomas Wiegand; Riccardo Cadalbert; Christine von Schroetter; Frédéric H-T Allain; Beat H Meier
Journal:  J Biomol NMR       Date:  2018-06-12       Impact factor: 2.835

2.  Solid-state NMR chemical-shift perturbations indicate domain reorientation of the DnaG primase in the primosome of Helicobacter pylori.

Authors:  Carole Gardiennet; Thomas Wiegand; Alexandre Bazin; Riccardo Cadalbert; Britta Kunert; Denis Lacabanne; Irina Gutsche; Laurent Terradot; Beat H Meier; Anja Böckmann
Journal:  J Biomol NMR       Date:  2016-03-10       Impact factor: 2.835

3.  Variability and conservation of structural domains in divide-and-conquer approaches.

Authors:  Thomas Wiegand; Carole Gardiennet; Riccardo Cadalbert; Denis Lacabanne; Britta Kunert; Laurent Terradot; Anja Böckmann; Beat H Meier
Journal:  J Biomol NMR       Date:  2016-05-30       Impact factor: 2.835

4.  On the use of ultracentrifugal devices for routine sample preparation in biomolecular magic-angle-spinning NMR.

Authors:  Abhishek Mandal; Jennifer C Boatz; Travis B Wheeler; Patrick C A van der Wel
Journal:  J Biomol NMR       Date:  2017-02-22       Impact factor: 2.835

5.  Bidirectional band-selective magnetization transfer along the protein backbone doubles the information content of solid-state NMR correlation experiments.

Authors:  M M Jolly; J A Jarvis; M Carravetta; M H Levitt; P T F Williamson
Journal:  J Biomol NMR       Date:  2017-11-08       Impact factor: 2.835

6.  Sedimentation Yields Long-Term Stable Protein Samples as Shown by Solid-State NMR.

Authors:  Thomas Wiegand; Denis Lacabanne; Anahit Torosyan; Julien Boudet; Riccardo Cadalbert; Frédéric H-T Allain; Beat H Meier; Anja Böckmann
Journal:  Front Mol Biosci       Date:  2020-02-21

Review 7.  ATP Analogues for Structural Investigations: Case Studies of a DnaB Helicase and an ABC Transporter.

Authors:  Denis Lacabanne; Thomas Wiegand; Nino Wili; Maria I Kozlova; Riccardo Cadalbert; Daniel Klose; Armen Y Mulkidjanian; Beat H Meier; Anja Böckmann
Journal:  Molecules       Date:  2020-11-12       Impact factor: 4.411

8.  Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution.

Authors:  Morgane Callon; Alexander A Malär; Sara Pfister; Václav Římal; Marco E Weber; Thomas Wiegand; Johannes Zehnder; Matías Chávez; Riccardo Cadalbert; Rajdeep Deb; Alexander Däpp; Marie-Laure Fogeron; Andreas Hunkeler; Lauriane Lecoq; Anahit Torosyan; Dawid Zyla; Rudolf Glockshuber; Stefanie Jonas; Michael Nassal; Matthias Ernst; Anja Böckmann; Beat H Meier
Journal:  J Biomol NMR       Date:  2021-06-25       Impact factor: 2.835
  8 in total

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