Hsiu-Ching Hsu1, Chia-Hsin Liu2, Yi-Chen Tsai2, Sin-Jin Li2, Ching-Yi Chen3, Chun-Han Chu2, Ming-Fong Chen1. 1. Department of Internal Medicine, National Taiwan University Hospital, 7 Chung-Shan S Rd, Taipei, Taiwan. 2. Department of Animal Science and Technology, National Taiwan University, 50, Lane 155, Sec 3, Keelung Rd, Taipei, 10672, Taiwan. 3. Department of Animal Science and Technology, National Taiwan University, No. 50, Lane 155, Sec 3, Keelung Rd, Taipei, 10672, Taiwan. ronichen@ntu.edu.tw.
Abstract
PURPOSE: Both endoplasmic reticulum stress (ER stress) and autophagy are essential for the response of the protein quality control system to cellular stresses. This study investigated the influence of the duration of a high-fat diet (HFD) in mice on tissue-specific cellular responses, specifically with regard to the role of autophagy and ER stress. METHODS: Male mice aged 6-7 weeks were fed ad libitum with a standard chow diet or with a HFD for 2, 4, 8, or 16 weeks. RESULTS: The HFD progressively increased mean body weight and induced tissue hypertrophy. The expression of PERK was suppressed in the liver after 16 weeks of the HFD and in the heart after 8 weeks of the HFD. Procaspase 12 and its activated form were induced in the liver with the HFD after 2 weeks, but not in the heart over the 16-week period. The activation of hepatic AMPK was elevated following 4 weeks of the HFD, but was inhibited after 16 weeks of the HFD. The ratio of LC3II to LC3I in the liver did not increase except in those mice fed the HFD for 16 weeks. The expression of AMPK and LC3 in the heart did not change over the entire 16 weeks of feeding the HFD. Cleaved PARP was increased in the liver and heart of mice receiving the HFD for 8 weeks. CONCLUSIONS: This study provides evidence that a HFD affects the cellular protein quality control processes responsible for metabolic disorder in a tissue- and duration-dependent manner.
PURPOSE: Both endoplasmic reticulum stress (ER stress) and autophagy are essential for the response of the protein quality control system to cellular stresses. This study investigated the influence of the duration of a high-fat diet (HFD) in mice on tissue-specific cellular responses, specifically with regard to the role of autophagy and ER stress. METHODS: Male mice aged 6-7 weeks were fed ad libitum with a standard chow diet or with a HFD for 2, 4, 8, or 16 weeks. RESULTS: The HFD progressively increased mean body weight and induced tissue hypertrophy. The expression of PERK was suppressed in the liver after 16 weeks of the HFD and in the heart after 8 weeks of the HFD. Procaspase 12 and its activated form were induced in the liver with the HFD after 2 weeks, but not in the heart over the 16-week period. The activation of hepatic AMPK was elevated following 4 weeks of the HFD, but was inhibited after 16 weeks of the HFD. The ratio of LC3II to LC3I in the liver did not increase except in those mice fed the HFD for 16 weeks. The expression of AMPK and LC3 in the heart did not change over the entire 16 weeks of feeding the HFD. Cleaved PARP was increased in the liver and heart of mice receiving the HFD for 8 weeks. CONCLUSIONS: This study provides evidence that a HFD affects the cellular protein quality control processes responsible for metabolic disorder in a tissue- and duration-dependent manner.
Authors: Sara B Cullinan; Donna Zhang; Mark Hannink; Edward Arvisais; Randal J Kaufman; J Alan Diehl Journal: Mol Cell Biol Date: 2003-10 Impact factor: 4.272
Authors: Luuk J Rijzewijk; Jacqueline T Jonker; Rutger W van der Meer; Mark Lubberink; Hugo W de Jong; Johannes A Romijn; Jeroen J Bax; Albert de Roos; Robert J Heine; Jos W Twisk; Albert D Windhorst; Adriaan A Lammertsma; Johannes W A Smit; Michaela Diamant; Hildo J Lamb Journal: J Am Coll Cardiol Date: 2010-07-13 Impact factor: 24.094
Authors: Deanna L Howarth; Claudia Lindtner; Ana M Vacaru; Ravi Sachidanandam; Orkhontuya Tsedensodnom; Taisa Vasilkova; Christoph Buettner; Kirsten C Sadler Journal: PLoS Genet Date: 2014-05-29 Impact factor: 5.917
Authors: Robert A Kloner; David A Brown; Marie Csete; Wangde Dai; James M Downey; Roberta A Gottlieb; Sharon L Hale; Jianru Shi Journal: Nat Rev Cardiol Date: 2017-07-27 Impact factor: 32.419
Authors: Ana Isabel Álvarez-Mercado; Carlos Rojano-Alfonso; Marc Micó-Carnero; Albert Caballeria-Casals; Carmen Peralta; Araní Casillas-Ramírez Journal: Front Cell Dev Biol Date: 2021-06-01
Authors: Jiaojiao Pang; Nathan D Fuller; Nan Hu; Linzi A Barton; Jeremy M Henion; Rui Guo; Yuguo Chen; Jun Ren Journal: PLoS One Date: 2016-01-25 Impact factor: 3.240
Authors: Maja M Dethlefsen; Caroline M Kristensen; Anna S Tøndering; Signe B Lassen; Stine Ringholm; Henriette Pilegaard Journal: Physiol Rep Date: 2018-07