Lian-Lian Si1, Lu Lv2, Wen-Hui Zhou3, Wei-Dong Hu2. 1. Department of Thoracic Oncology, Zhongnan Hospital of Wuhan University, Wuhan University Wuhan, Hubei 430071, China ; Department of People's Hospital of Beijing Daxing District Beijing 102600, China. 2. Department of Thoracic Oncology, Zhongnan Hospital of Wuhan University, Wuhan University Wuhan, Hubei 430071, China ; Hubei Cancer Clinical Study Center & Hubei Key Laboratory of Tumor Biological Behaviors Wuhan, Hubei 430071, China. 3. Medical Center for Human Reproduction, Beijing Chaoyang Hospital, Capital Medical University Beijing 100020, China.
Abstract
OBJECTIVE: To explore a simple and practical method for human primary lung cancer cells culture in vitro. METHODS: Tumor specimens from 6 lung cancer patients were isolated with collagenase digestion cultured in vitro. Then the characteristics of these cells were analyzed and identified by optical microscope observation, hematoxylin-eosin staining, immunocytochemistry, immunohistochemistry and tumor nude mice inoculation experiments, respectively. RESULTS: Except for the small cell lung cancer, the other 5 samples were successfully isolated and cultured. The cultured cells showed typical characteristics of malignant cells and positive for cytokeratin 7 and 19. Moreover, the cancer cells readily formed subcutaneous tumors in nude mice and the pathological images of the transplanted tumor were consistent with its tumor origin. CONCLUSION: The primary culture for human lung cancer cells can be successfully achieved with the method of collagenase digestion.
OBJECTIVE: To explore a simple and practical method for human primary lung cancer cells culture in vitro. METHODS:Tumor specimens from 6 lung cancerpatients were isolated with collagenase digestion cultured in vitro. Then the characteristics of these cells were analyzed and identified by optical microscope observation, hematoxylin-eosin staining, immunocytochemistry, immunohistochemistry and tumornude mice inoculation experiments, respectively. RESULTS: Except for the small cell lung cancer, the other 5 samples were successfully isolated and cultured. The cultured cells showed typical characteristics of malignant cells and positive for cytokeratin 7 and 19. Moreover, the cancer cells readily formed subcutaneous tumors in nude mice and the pathological images of the transplanted tumor were consistent with its tumor origin. CONCLUSION: The primary culture for humanlung cancer cells can be successfully achieved with the method of collagenase digestion.