AIM: To establish and characterize primary lung cancer cell lines from Chinese population. METHODS: Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice. RESULTS: Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations. CONCLUSION: These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response.
AIM: To establish and characterize primary lung cancer cell lines from Chinese population. METHODS:Lung cancer specimens or pleural effusions were collected from Chinese lung cancerpatients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice. RESULTS: Eight primary cell lines from Chinese lung cancerpatients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations. CONCLUSION: These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response.
Authors: Krijn K Dijkstra; Chiara M Cattaneo; Fleur Weeber; Myriam Chalabi; Joris van de Haar; Lorenzo F Fanchi; Maarten Slagter; Daphne L van der Velden; Sovann Kaing; Sander Kelderman; Nienke van Rooij; Monique E van Leerdam; Annekatrien Depla; Egbert F Smit; Koen J Hartemink; Rosa de Groot; Monika C Wolkers; Norman Sachs; Petur Snaebjornsson; Kim Monkhorst; John Haanen; Hans Clevers; Ton N Schumacher; Emile E Voest Journal: Cell Date: 2018-08-09 Impact factor: 41.582
Authors: S Michael Rothenberg; Kyle Concannon; Sarah Cullen; Gaylor Boulay; Alexa B Turke; Anthony C Faber; Elizabeth L Lockerman; Miguel N Rivera; Jeffrey A Engelman; Shyamala Maheswaran; Daniel A Haber Journal: Elife Date: 2015-02-16 Impact factor: 8.140