| Literature DB >> 26253969 |
Dan Dominissini1, Sharon Moshitch-Moshkovitz2, Ninette Amariglio2, Gideon Rechavi3.
Abstract
A detailed protocol for isolation and sequencing of an enriched population of m(6)A-methylated RNA fragments to create m(6)A methylome maps is outlined. Our approach was developed to fill a void that existed because of a lack of methods for the detection of m(6)A in RNA in an unbiased, high-throughput, and high-resolution manner. This method integrates immunoprecipitation of methylated, randomly fragmented RNA using a highly specific anti-m(6)A antibody to obtain an enriched population of modified fragments and massively parallel sequencing, resulting in mapping of this modification throughout the transcriptome.Entities:
Keywords: Immunocapturing; N(6)-Methyladenosine; Next-generation sequencing; RNA immunoprecipitation; RNA methylation
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Year: 2015 PMID： 26253969 DOI： 10.1016/bs.mie.2015.03.001
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600