Xujie Liu1,2, Shengjun Shi3, Qingling Feng2,4, Akash Bachhuka5, Wei He2, Qianli Huang2, Ranran Zhang2, Xing Yang2, Krasimir Vasilev5,6. 1. Graduate School at Shenzhen, Tsinghua University , Shenzhen 518055, China. 2. State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China. 3. The Burns Department of Zhujiang Hospital, Southern Medical University , Guangzhou 510280, China. 4. Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University , Beijing 100084, China. 5. Mawson Institute, University of South Australia , Mawson Lakes 5095, Australia. 6. School of Advanced Manufacturing, University of South Australia , Mawson Lakes 5095, Australia.
Abstract
To understand the role of surface chemistry on cell behavior and the associated molecular mechanisms, we developed and utilized a surface chemical gradient of amine functional groups by carefully adjusting the gas composition of 1,7-octadiene (OD) and allylamine (AA) of the plasma phase above a moving substrate. The chemical gradient surface used in the present work shows an increasing N/C ratio and wettability from the OD side toward the AA side with no change in surface topography. Under standard culture conditions (with serum), human adipose-derived stem cells (hASCs) adhesion and spreading area increased toward the AA side of the gradient. However, there were no differences in cell behavior in the absence of serum. These results, supported by the trends in proteins adsorption on the gradient surface, demonstrated that surface chemistry affects the response of hASCs through cell-adhesive serum proteins, rather than interacting directly with the cells. The expression of p-ERK and the osteogenic differentiation increased toward the AA side of the gradient, while adipogenic differentiation decreased in the same direction; however, when the activation of ERK1/2 was blocked by PD98059, the levels of osteogenic or adipogenic differentiation on different regions of the chemical gradient were the same. This indicates that ERK1/2 may be an important downstream signaling pathway of surface chemistry directed stem cell fate.
To understand the role of surface chemistry on cell behavior and the associated molecular mechanisms, we developed and utilized a surface chemical gradient of amine functional groups by carefully adjusting the gas composition of n class="Chemical">1,7-octadiene (OD) and allylamine (AA) of the plasma phase above a moving substrate. The chemical gradient surface used in the present work shows an increasing N/C ratio and wettability from the OD side toward the AA side with no change in surface topography. Under standard culture conditions (with serum), human adipose-derived stem cells (hASCs) adhesion and spreading area increased toward the AA side of the gradient. However, there were no differences in cell behavior in the absence of serum. These results, supported by the trends in proteins adsorption on the gradient surface, demonstrated that surface chemistry affects the response of hASCs through cell-adhesive serum proteins, rather than interacting directly with the cells. The expression of p-ERK and the osteogenic differentiation increased toward the AA side of the gradient, while adipogenic differentiation decreased in the same direction; however, when the activation of ERK1/2 was blocked by PD98059, the levels of osteogenic or adipogenic differentiation on different regions of the chemical gradient were the same. This indicates that ERK1/2 may be an important downstream signaling pathway of surface chemistry directed stem cell fate.
Authors: Thomas C Day; Pedro Márquez-Zacarías; Pablo Bravo; Aawaz R Pokhrel; Kathryn A MacGillivray; William C Ratcliff; Peter J Yunker Journal: Biophys Rev (Melville) Date: 2022-06-01
Authors: William J Hadden; Jennifer L Young; Andrew W Holle; Meg L McFetridge; Du Yong Kim; Philip Wijesinghe; Hermes Taylor-Weiner; Jessica H Wen; Andrew R Lee; Karen Bieback; Ba-Ngu Vo; David D Sampson; Brendan F Kennedy; Joachim P Spatz; Adam J Engler; Yu Suk Choi Journal: Proc Natl Acad Sci U S A Date: 2017-05-15 Impact factor: 11.205