Literature DB >> 26237569

In Situ Hybridization to Identify Gut Stem Cells.

Alex Gregorieff1, Hans Clevers1.   

Abstract

In recent years, considerable effort has been directed toward identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.
Copyright © 2015 John Wiley & Sons, Inc.

Keywords:  (ISH) in situ hybridization; digoxigenin RNA probes; formalin-fixed paraffin-embedded; intestinal sections

Mesh：

Year:  2015        PMID： 26237569     DOI： 10.1002/9780470151808.sc02f01s34

Source DB:  PubMed          Journal:  Curr Protoc Stem Cell Biol        ISSN： 1938-8969


  9 in total

Review 1.  The Role of the p53 Protein in Stem-Cell Biology and Epigenetic Regulation.

Authors:  Arnold J Levine; Anna M Puzio-Kuter; Chang S Chan; Pierre Hainaut
Journal:  Cold Spring Harb Perspect Med       Date:  2016-09-01       Impact factor: 6.915

2.  A unique role of thyroid hormone receptor β in regulating notochord resorption during Xenopus metamorphosis.

Authors:  Keisuke Nakajima; Ichiro Tazawa; Yun-Bo Shi
Journal:  Gen Comp Endocrinol       Date:  2019-03-06       Impact factor: 2.822

3.  Pitx2-Sox2-Lef1 interactions specify progenitor oral/dental epithelial cell signaling centers.

Authors:  Wenjie Yu; Zhao Sun; Yan Sweat; Mason Sweat; Shankar Rengasamy Venugopalan; Steven Eliason; Huojun Cao; Michael L Paine; Brad A Amendt
Journal:  Development       Date:  2020-06-04       Impact factor: 6.868

4.  Sox2 and Lef-1 interact with Pitx2 to regulate incisor development and stem cell renewal.

Authors:  Zhao Sun; Wenjie Yu; Maria Sanz Navarro; Mason Sweat; Steven Eliason; Thad Sharp; Huan Liu; Kerstin Seidel; Li Zhang; Myriam Moreno; Thomas Lynch; Nathan E Holton; Laura Rogers; Traci Neff; Michael J Goodheart; Frederic Michon; Ophir D Klein; Yang Chai; Adam Dupuy; John F Engelhardt; Zhi Chen; Brad A Amendt
Journal:  Development       Date:  2016-09-22       Impact factor: 6.868

5.  Genomic analyses in African populations identify novel risk loci for cleft palate.

Authors:  Azeez Butali; Peter A Mossey; Wasiu L Adeyemo; Mekonen A Eshete; Lord J J Gowans; Tamara D Busch; Deepti Jain; Wenjie Yu; Liu Huan; Cecelia A Laurie; Cathy C Laurie; Sarah Nelson; Mary Li; Pedro A Sanchez-Lara; William P Magee; Kathleen S Magee; Allyn Auslander; Frederick Brindopke; Denise M Kay; Michele Caggana; Paul A Romitti; James L Mills; Rosemary Audu; Chika Onwuamah; Ganiyu O Oseni; Arwa Owais; Olutayo James; Peter B Olaitan; Babatunde S Aregbesola; Ramat O Braimah; Fadekemi O Oginni; Ayodeji O Oladele; Saidu A Bello; Jennifer Rhodes; Rita Shiang; Peter Donkor; Solomon Obiri-Yeboah; Fareed Kow Nanse Arthur; Peter Twumasi; Pius Agbenorku; Gyikua Plange-Rhule; Alexander Acheampong Oti; Olugbenga M Ogunlewe; Afisu A Oladega; Adegbayi A Adekunle; Akinwunmi O Erinoso; Olatunbosun O Adamson; Abosede A Elufowoju; Oluwanifemi I Ayelomi; Taiye Hailu; Abiye Hailu; Yohannes Demissie; Miliard Derebew; Steve Eliason; Miguel Romero-Bustillous; Cynthia Lo; James Park; Shaan Desai; Muiawa Mohammed; Firke Abate; Lukman O Abdur-Rahman; Deepti Anand; Irfaan Saadi; Abimibola V Oladugba; Salil A Lachke; Brad A Amendt; Charles N Rotimi; Mary L Marazita; Robert A Cornell; Jeffrey C Murray; Adebowale A Adeyemo
Journal:  Hum Mol Genet       Date:  2019-03-15       Impact factor: 6.150

Review 6.  Intestinal Stem Cell Niche Insights Gathered from Both In Vivo and Novel In Vitro Models.

Authors:  Nikolce Gjorevski; Paloma Ordóñez-Morán
Journal:  Stem Cells Int       Date:  2017-09-07       Impact factor: 5.443

7.  Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche.

Authors:  Ysbrand M Nusse; Adam K Savage; Pauline Marangoni; Axel K M Rosendahl-Huber; Tyler A Landman; Frederic J de Sauvage; Richard M Locksley; Ophir D Klein
Journal:  Nature       Date:  2018-06-27       Impact factor: 69.504

8.  Missense Pathogenic variants in KIF4A Affect Dental Morphogenesis Resulting in X-linked Taurodontism, Microdontia and Dens-Invaginatus.

Authors:  Lord J J Gowans; Sophia Cameron-Christie; Rebecca L Slayton; Tamara Busch; Miguel Romero-Bustillos; Steven Eliason; Mason Sweat; Nara Sobreira; Wenjie Yu; Piranit N Kantaputra; Elizabeth Wohler; Wasiu Lanre Adeyemo; Salil A Lachke; Deepti Anand; Collen Campbell; Bernadette K Drummond; David M Markie; W Jansen van Vuuren; L Jansen van Vuuren; Paul S Casamassimo; Ronald Ettinger; Arwa Owais; I van Staden; Brad A Amendt; Adebowale A Adeyemo; Jeffrey C Murray; Stephen P Robertson; Azeez Butali
Journal:  Front Genet       Date:  2019-09-20       Impact factor: 4.599

9.  MLL1 is required for maintenance of intestinal stem cells.

Authors:  Neha Goveas; Claudia Waskow; Kathrin Arndt; Julian Heuberger; Qinyu Zhang; Dimitra Alexopoulou; Andreas Dahl; Walter Birchmeier; Konstantinos Anastassiadis; A Francis Stewart; Andrea Kranz
Journal:  PLoS Genet       Date:  2021-12-03       Impact factor: 5.917
  9 in total

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