| Literature DB >> 26237057 |
Chul Woo Min1, Ravi Gupta1, So Wun Kim1, So Eui Lee1, Yong Chul Kim1, Dong Won Bae2, Won Young Han3, Byong Won Lee3, Jong Min Ko3, Ganesh Kumar Agrawal4,5, Randeep Rakwal4,5, Sun Tae Kim1.
Abstract
This study develops differential protein profiles of soybean (Glycine max) seeds (cv. Saedanbaek and Daewon) varying in protein (47.9 and 39.2%) and oil (16.3 and 19.7%) content using protamine sulfate (PS) precipitation method coupled with a 2D gel electrophoresis (2DGE) approach. Of 71 detected differential spots between Daewon and Saedanbaek, 48 were successfully identified by MALDI-TOF/TOF. Gene ontology analysis revealed that up-regulated proteins in Saedanbaek were largely associated with nutrient reservoir activity (42.6%), which included mainly seed-storage proteins (SSPs; subunits of glycinin and β-conglycinin). Similar results were also obtained in two cultivars of wild soybean (G. soja cv. WS22 and WS15) differing in protein content. Western blots confirmed higher accumulation of SSPs in protein-rich Saedanbaek. Findings presented and discussed in this study highlight a possible involvement of the urea cycle for increased accumulation of SSPs and hence the higher protein content in soybean seeds.Entities:
Keywords: 2D gel electrophoresis; Glycine max; Glycine soja; MALDI-TOF/TOF; protamine sulfate precipitation method; seed-storage proteins
Mesh：
Substances：
Year: 2015 PMID： 26237057 DOI： 10.1021/acs.jafc.5b03196
Source DB: PubMed Journal: J Agric Food Chem ISSN： 0021-8561 Impact factor: 5.279