Literature DB >> 26236790

Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd(3+)-DOTA.

Filip Wojciechowski1, Andreas Groß, Isabelle T Holder, Laura Knörr, Malte Drescher, Jörg S Hartig.   

Abstract

Gd(3+) is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd(3+)-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels.

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Year:  2015        PMID： 26236790     DOI： 10.1039/c5cc04234h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  6 in total

1.  Xenopus laevis Oocytes Preparation for in-Cell EPR Spectroscopy.

Authors:  Laura John; Malte Drescher
Journal:  Bio Protoc       Date:  2018-04-05

2.  Cleavage-Resistant Protein Labeling With Hydrophilic Trityl Enables Distance Measurements In-Cell.

Authors:  Zikri Hasanbasri; Kevin Singewald; Teresa D Gluth; Benoit Driesschaert; Sunil Saxena
Journal:  J Phys Chem B       Date:  2021-05-13       Impact factor: 3.466

3.  SLIM: A Short-Linked, Highly Redox-Stable Trityl Label for High-Sensitivity In-Cell EPR Distance Measurements.

Authors:  Nico Fleck; Caspar A Heubach; Tobias Hett; Florian R Haege; Pawel P Bawol; Helmut Baltruschat; Olav Schiemann
Journal:  Angew Chem Int Ed Engl       Date:  2020-04-30       Impact factor: 15.336

4.  Site-Directed Spin Labeling of RNA with a Gem-Diethylisoindoline Spin Label: PELDOR, Relaxation, and Reduction Stability.

Authors:  Christine Wuebben; Simon Blume; Dinar Abdullin; Dominik Brajtenbach; Florian Haege; Stephanie Kath-Schorr; Olav Schiemann
Journal:  Molecules       Date:  2019-12-06       Impact factor: 4.411

5.  Cu2+-based distance measurements by pulsed EPR provide distance constraints for DNA backbone conformations in solution.

Authors:  Shreya Ghosh; Matthew J Lawless; Hanna J Brubaker; Kevin Singewald; Michael R Kurpiewski; Linda Jen-Jacobson; Sunil Saxena
Journal:  Nucleic Acids Res       Date:  2020-05-21       Impact factor: 16.971

6.  Compaction of RNA Duplexes in the Cell*.

Authors:  Alberto Collauto; Sören von Bülow; Dnyaneshwar B Gophane; Subham Saha; Lukas S Stelzl; Gerhard Hummer; Snorri T Sigurdsson; Thomas F Prisner
Journal:  Angew Chem Int Ed Engl       Date:  2020-10-13       Impact factor: 15.336
  6 in total

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