Lorenzo Drago1,2, Elena De Vecchi1, Monica Bortolin1, Marco Toscano1, Roberto Mattina3, Carlo Luca Romanò4. 1. Laboratory of Clinical Chemistry & Microbiology, IRCCS Galeazzi Orthopaedic Institute, via R Galeazzi 4, 20161 Milan, Italy. 2. Laboratory of Technical Sciences for Laboratory Medicine, Department of Biomedical Science for Health, University of Milan, via L Mangiagalli 31, 20133 Milan, Italy. 3. Department of Public Health, Microbiology & Virology, University of Milan, via C Pascal 36, 20133 Milan, Italy. 4. Centre for Reconstructive Surgery & Bone Infections, IRCCS Galeazzi Orthopaedic Institute, via R Galeazzi 4, 20161 Milan, Italy.
Abstract
AIMS: This study aimed to evaluate the antimicrobial activity of two different formulations of bioglass BAG-S53P4 against multiresistant microorganisms involved in bone infections, and the capability of bioglass to select for resistance. METHODS: Antibacterial activity was evaluated by means of killing curves. The ability to select for resistant bacteria was evaluated by subculturing microorganisms in serial dilutions of bioglass. Scanning electron microscope acquisitions were conducted to evaluate bioglass-induced morphology changes. RESULTS: BAG-S53P4 formulations display a high antimicrobial activity and do not seem to select for resistance. Scanning electron microscopy analysis showed cell shrinkage and membrane damage after exposure to bioglass. CONCLUSIONS: BAG-S53P4 has a significant potential as bone substitute for the treatment of infections caused by multiresistant microorganisms.
AIMS: This study aimed to evaluate the antimicrobial activity of two different formulations of bioglass BAG-S53P4 against multiresistant microorganisms involved in bone infections, and the capability of bioglass to select for resistance. METHODS: Antibacterial activity was evaluated by means of killing curves. The ability to select for resistant bacteria was evaluated by subculturing microorganisms in serial dilutions of bioglass. Scanning electron microscope acquisitions were conducted to evaluate bioglass-induced morphology changes. RESULTS: BAG-S53P4 formulations display a high antimicrobial activity and do not seem to select for resistance. Scanning electron microscopy analysis showed cell shrinkage and membrane damage after exposure to bioglass. CONCLUSIONS: BAG-S53P4 has a significant potential as bone substitute for the treatment of infections caused by multiresistant microorganisms.
Entities:
Keywords:
antibacterial activity; bioactive glass S53P4; bone infection; multidrug resistance; scanning electron microscopy; selection of resistance
Authors: Sergio Rius-Rocabert; Javier Arranz-Herrero; Adolfo Fernández-Valdés; Marzia Marciello; Sandra Moreno; Francisco Llinares-Pinel; Jesus Presa; Rubén Hernandez-Alcoceba; Roberto López-Píriz; Ramón Torrecillas; Antonia García; Alejandro Brun; Marco Filice; José S Moya; Belen Cabal; Estanislao Nistal-Villan Journal: Mater Today Bio Date: 2021-12-22
Authors: Lisa Kirchhoff; Diana Arweiler-Harbeck; Judith Arnolds; Timon Hussain; Stefan Hansen; Ralph Bertram; Jan Buer; Stephan Lang; Joerg Steinmann; Benedikt Höing Journal: PLoS One Date: 2020-02-21 Impact factor: 3.240