OBJECTIVE: In this study, we investigated the role of miR-300 in regulating cell proliferation and invasion of gastric cancer cells. METHODS: MicroRNA and protein expression patterns were compared between gastric cancer tissue and normal tissue and between two different prognostic groups. The up-regulation of miR-300 was confirmed by real-time reverse transcription polymerase chain reaction and its expression was analyzed in AGS gastric cancer cells. RESULTS: We observed that miR-300 expression was frequently and dramatically up-regulated in human gastric cancer tissues and cell lines compared with the matched adjacent normal tissues and cells. We further showed that transient and stable over-expression of miR-300 could promote cell proliferation and cell cycle progression. Moreover, p53, a key inhibitor of cell cycle, was verified as a direct target of miR-300, suggesting that miR-300 might promote gastric cancer cell proliferation and invasion by increasing p53 expression. CONCLUSION: Our findings indicated that miR-300 up-regulation might exert some sort of antagonistic function by targeting p53 in gastric cancer cell proliferation during gastric tumorigenesis.
OBJECTIVE: In this study, we investigated the role of miR-300 in regulating cell proliferation and invasion of gastric cancer cells. METHODS: MicroRNA and protein expression patterns were compared between gastric cancer tissue and normal tissue and between two different prognostic groups. The up-regulation of miR-300 was confirmed by real-time reverse transcription polymerase chain reaction and its expression was analyzed in AGS gastric cancer cells. RESULTS: We observed that miR-300 expression was frequently and dramatically up-regulated in humangastric cancer tissues and cell lines compared with the matched adjacent normal tissues and cells. We further showed that transient and stable over-expression of miR-300 could promote cell proliferation and cell cycle progression. Moreover, p53, a key inhibitor of cell cycle, was verified as a direct target of miR-300, suggesting that miR-300 might promote gastric cancer cell proliferation and invasion by increasing p53 expression. CONCLUSION: Our findings indicated that miR-300 up-regulation might exert some sort of antagonistic function by targeting p53 in gastric cancer cell proliferation during gastric tumorigenesis.
Authors: Azhar Rasul; Muhammad Khan; Bo Yu; Muhammad Ali; Yang Jing Bo; Hong Yang; Tonghui Ma Journal: Arch Pharm Res Date: 2013-07-24 Impact factor: 4.946
Authors: Giuseppe Aprile; Riccardo Giampieri; Marta Bonotto; Alessandro Bittoni; Elena Ongaro; Giovanni Gerardo Cardellino; Francesco Graziano; Francesco Giuliani; Gianpiero Fasola; Stefano Cascinu; Mario Scartozzi Journal: Expert Opin Investig Drugs Date: 2014-05-07 Impact factor: 6.206