Ya-Min Liu1, Qiu-Ying Xu1, Jun-Xian Chen2, Song Chen3, Hao-Jun Peng1, Zheng-Lun Zeng1, Zhi-Yu Feng1, Qiang Shen1. 1. The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine Guangzhou 510405, China. 2. ChangGang Street Community Health Service Center Haizhu District, Guangzhou 510280, China. 3. Tropical Medicine Institute, Guangzhou University of Chinese Medicine Guangzhou 510405, China.
Abstract
OBJECTIVE: To study the immune mechanism of nourishing kidney and eliminating toxicity decoction (NKETD) on Chronic Hepatitis B (CHB), we detected the serum concentrations of IFN-γ (the characteristic cytokine of Th1), IL-17A (the characteristic cytokine of Th17) and the quantitative proportion of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg in HBV transgenic mice. METHODS: The HBV transgenic mice were randomly divided into six groups: high-dose group, middle-dose group, low-dose group, lamivudine group, model control group and normal mice control group. The serum concentrations of IFN-γ and IL-17A in mice were measured by ELISA method and the ratio of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg was detected by Flow Cytometry Method (FCM). RESULTS: The decoction could increase the serum concentration of IFN-γ and decrease that of IL-17A in HBV transgenic mice. The higher the dose was, the more significantly the concentration of IFN-γ increased. And high-dose decoction could decrease the serum concentration of IL-17A in HBV transgenic mice significantly and continuously while middle-dose and low-dose decoction had no significant effects. However, there wasn't statistically significant variation on the ratio of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg in HBV transgenic mice. CONCLUSION: The decoction could treat CHB by regulating the immune function by promoting the generation of Th1 and/or enhancing its function while inhibiting Th17. The immune regulation by decoction had more significant effects than lamivudine.
OBJECTIVE: To study the immune mechanism of nourishing kidney and eliminating toxicity decoction (NKETD) on Chronic Hepatitis B (CHB), we detected the serum concentrations of IFN-γ (the characteristic cytokine of Th1), IL-17A (the characteristic cytokine of Th17) and the quantitative proportion of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg in HBV transgenic mice. METHODS: The HBV transgenic mice were randomly divided into six groups: high-dose group, middle-dose group, low-dose group, lamivudine group, model control group and normal mice control group. The serum concentrations of IFN-γ and IL-17A in mice were measured by ELISA method and the ratio of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg was detected by Flow Cytometry Method (FCM). RESULTS: The decoction could increase the serum concentration of IFN-γ and decrease that of IL-17A in HBV transgenic mice. The higher the dose was, the more significantly the concentration of IFN-γ increased. And high-dose decoction could decrease the serum concentration of IL-17A in HBV transgenic mice significantly and continuously while middle-dose and low-dose decoction had no significant effects. However, there wasn't statistically significant variation on the ratio of CD(+) 4CD(+) 25 foxp3 Treg to CD(+) 4 Treg in HBV transgenic mice. CONCLUSION: The decoction could treat CHB by regulating the immune function by promoting the generation of Th1 and/or enhancing its function while inhibiting Th17. The immune regulation by decoction had more significant effects than lamivudine.
Entities:
Keywords:
Chronic Hepatitis B; IFN-γ; IL-17A; Nourishing kidney and eliminating toxicity decoction; ratio of CD+4CD+25 foxp3 Treg to CD+4 Treg
Authors: A Penna; G Del Prete; A Cavalli; A Bertoletti; M M D'Elios; R Sorrentino; M D'Amato; C Boni; M Pilli; F Fiaccadori; C Ferrari Journal: Hepatology Date: 1997-04 Impact factor: 17.425
Authors: Rui Guo; Yi Tian; Xueyuan Jin; Haiyan Chen; Guihu Wang; Xiaozhong Huang; Burong Li; Zongfang Li; Jun Yang Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2019-09-30