Kyung-Won Hong1,2, Myung-Seop Shin3, Yoo-Been Ahn3, Hyun-Jin Lee3, Hyun-Duck Kim3,4. 1. Theragen Etex Bio Institute, Suwonsi, Gyeonggi-do, Korea. 2. Division of Biomedical Informatics, Center for Genome Science, National Institute of Health, KCDC, Cheongju-si, Chungbuk, Korea. 3. Department of Preventive and Social Dentistry, School of Dentistry, Seoul National University, Daehak-ro, Seoul, Korea. 4. Dental Research Institute, School of Dentistry, Seoul National University, Daehak-ro, Seoul, Korea.
Abstract
AIM: This study aims to locate the genes related to periodontitis through genome-wide association study (GWAS) in Korean population. METHODS: Total of 677 adults aged 44-88 years were recruited from the Yangpyeong cohort in Korea. The participants did not have self-reported metabolic diseases, cardiovascular diseases or cancer. Periodontitis was assessed using alveolar bone loss from a digital panoramic radiograph and classified into three groups: normal to mild, moderate and severe periodontitis. DNA from blood samples were genotyped using the Illumina Human 1M-duo Beadchip. Multivariable logistic regression analysis in PLINK was applied to examine the single-nucleotide polymorphisms (SNPs) related to periodontitis after controlling for various confounders. RESULTS: Associations of three SNPs suggested TENM2 (rs4242220) and LDLRAD4 (rs12969041, rs2027756) as putative risk genes of chronic periodontitis (p-values <1 × 10-5 ). The odds ratio [95% confidence interval (CI)] of TENM2 was 0.53 (0.40-0.70) for moderate periodontitis and that of LDLRAD4 was 2.86 (1.92-4.27) for severe periodontitis. Two nonsynonymous SNPs of protein coding region and seven SNPs selected from previous reports showed nominal association. CONCLUSION: Our GWAS supports a previously reported gene of TENM2 and newly suggests LDLRAD4. These two genes' role on lipid metabolism may play a part in the molecular aetiology of periodontitis.
AIM: This study aims to locate the genes related to periodontitis through genome-wide association study (GWAS) in Korean population. METHODS: Total of 677 adults aged 44-88 years were recruited from the Yangpyeong cohort in Korea. The participants did not have self-reported metabolic diseases, cardiovascular diseases or cancer. Periodontitis was assessed using alveolar bone loss from a digital panoramic radiograph and classified into three groups: normal to mild, moderate and severe periodontitis. DNA from blood samples were genotyped using the Illumina Human 1M-duo Beadchip. Multivariable logistic regression analysis in PLINK was applied to examine the single-nucleotide polymorphisms (SNPs) related to periodontitis after controlling for various confounders. RESULTS: Associations of three SNPs suggested TENM2 (rs4242220) and LDLRAD4 (rs12969041, rs2027756) as putative risk genes of chronic periodontitis (p-values <1 × 10-5 ). The odds ratio [95% confidence interval (CI)] of TENM2 was 0.53 (0.40-0.70) for moderate periodontitis and that of LDLRAD4 was 2.86 (1.92-4.27) for severe periodontitis. Two nonsynonymous SNPs of protein coding region and seven SNPs selected from previous reports showed nominal association. CONCLUSION: Our GWAS supports a previously reported gene of TENM2 and newly suggests LDLRAD4. These two genes' role on lipid metabolism may play a part in the molecular aetiology of periodontitis.
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