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Literature DB >> 26198865

Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media.

Seth T Housman¹, Mary Anne Banevicius¹, Lucinda M Lamb¹, David P Nicolau².

Abstract

We evaluated the isolation and quantitation of Clostridium difficile from aqueous and fecal samples utilizing ChromID CDIF and cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate media. Growth was similar between the two. ChromID CDIF provided enhanced isolation and required no ethanol pretreatment to inhibit normal flora. ChromID CDIF also improved turn-around time, requiring only 24 hours' incubation.

Entities: Chemical Species

Keywords: Chromogenic agar; Clostridium difficile; Cycloserine, cefoxitin, and fructose-containing agar with horse blood and taurocholate

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Year: 2015 PMID： 26198865 DOI： 10.1016/j.jmii.2015.05.012

Source DB: PubMed Journal: J Microbiol Immunol Infect ISSN： 1684-1182 Impact factor: 4.399

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Cited

3 in total

Isolation and quantitation of Clostridium difficile in aqueous and fecal matter using two types of selective media.

Review 1. Laboratory Tests for the Diagnosis of Clostridium difficile.

2. An exploratory study to evaluate Clostridium difficile polymerase chain reaction ribotypes and infection outcomes.

3. High prevalence of toxigenic Clostridium difficile in public space lawns in Western Australia.