Literature DB >> 26192319

Engineered CHO cells for production of diverse, homogeneous glycoproteins.

Zhang Yang1, Shengjun Wang1, Adnan Halim1, Morten Alder Schulz2, Morten Frodin3, Shamim H Rahman4, Malene B Vester-Christensen4, Carsten Behrens5, Claus Kristensen2, Sergey Y Vakhrushev2, Eric Paul Bennett2, Hans H Wandall2, Henrik Clausen1.   

Abstract

Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase genes controlling N-glycosylation in CHO cells and constructed a design matrix that facilitates the generation of desired glycosylation, such as human-like α2,6-linked sialic acid capping. This engineering approach will aid the production of glycoproteins with improved properties and therapeutic potential.

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Year:  2015        PMID： 26192319     DOI： 10.1038/nbt.3280

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN： 1087-0156            Impact factor:   54.908


  20 in total

Review 1.  Lectin-resistant CHO glycosylation mutants.

Authors:  Santosh Kumar Patnaik; Pamela Stanley
Journal:  Methods Enzymol       Date:  2006       Impact factor: 1.600

2.  Biopharmaceutical benchmarks 2014.

Authors:  Gary Walsh
Journal:  Nat Biotechnol       Date:  2014-10       Impact factor: 54.908

Review 3.  Glycoengineering: the effect of glycosylation on the properties of therapeutic proteins.

Authors:  Angus M Sinclair; Steve Elliott
Journal:  J Pharm Sci       Date:  2005-08       Impact factor: 3.534

4.  Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies.

Authors:  Laetitia Malphettes; Yevgeniy Freyvert; Jennifer Chang; Pei-Qi Liu; Edmond Chan; Jeffrey C Miller; Zhe Zhou; Thu Nguyen; Christina Tsai; Andrew W Snowden; Trevor N Collingwood; Philip D Gregory; Gregory J Cost
Journal:  Biotechnol Bioeng       Date:  2010-08-01       Impact factor: 4.530

5.  GlycoPEGylation of recombinant therapeutic proteins produced in Escherichia coli.

Authors:  Shawn DeFrees; Zhi-Guang Wang; Ruye Xing; Arthur E Scott; Jin Wang; David Zopf; Dominique L Gouty; Eric R Sjoberg; Krishnasamy Panneerselvam; Els C M Brinkman-Van der Linden; Robert J Bayer; Mads A Tarp; Henrik Clausen
Journal:  Glycobiology       Date:  2006-05-22       Impact factor: 4.313

6.  Site-specific glycosylation of human recombinant erythropoietin: analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry.

Authors:  H Sasaki; N Ochi; A Dell; M Fukuda
Journal:  Biochemistry       Date:  1988-11-15       Impact factor: 3.162

7.  Obligate ligation-gated recombination (ObLiGaRe): custom-designed nuclease-mediated targeted integration through nonhomologous end joining.

Authors:  Marcello Maresca; Victor Guosheng Lin; Ning Guo; Yi Yang
Journal:  Genome Res       Date:  2012-11-14       Impact factor: 9.043

8.  The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.

Authors:  Xun Xu; Harish Nagarajan; Nathan E Lewis; Shengkai Pan; Zhiming Cai; Xin Liu; Wenbin Chen; Min Xie; Wenliang Wang; Stephanie Hammond; Mikael R Andersen; Norma Neff; Benedetto Passarelli; Winston Koh; H Christina Fan; Jianbin Wang; Yaoting Gui; Kelvin H Lee; Michael J Betenbaugh; Stephen R Quake; Iman Famili; Bernhard O Palsson; Jun Wang
Journal:  Nat Biotechnol       Date:  2011-07-31       Impact factor: 54.908

9.  Fast and sensitive detection of indels induced by precise gene targeting.

Authors:  Zhang Yang; Catharina Steentoft; Camilla Hauge; Lars Hansen; Allan Lind Thomsen; Francesco Niola; Malene B Vester-Christensen; Morten Frödin; Henrik Clausen; Hans H Wandall; Eric P Bennett
Journal:  Nucleic Acids Res       Date:  2015-03-09       Impact factor: 16.971

10.  Glycomics profiling of Chinese hamster ovary cell glycosylation mutants reveals N-glycans of a novel size and complexity.

Authors:  Simon J North; Hung-Hsiang Huang; Subha Sundaram; Jihye Jang-Lee; A Tony Etienne; Alana Trollope; Sara Chalabi; Anne Dell; Pamela Stanley; Stuart M Haslam
Journal:  J Biol Chem       Date:  2009-12-01       Impact factor: 5.157
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  68 in total

1.  A Markov chain model for N-linked protein glycosylation--towards a low-parameter tool for model-driven glycoengineering.

Authors:  Philipp N Spahn; Anders H Hansen; Henning G Hansen; Johnny Arnsdorf; Helene F Kildegaard; Nathan E Lewis
Journal:  Metab Eng       Date:  2015-10-29       Impact factor: 9.783

2.  An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells.

Authors:  Yoshiki Narimatsu; Hiren J Joshi; Rebecca Nason; Julie Van Coillie; Richard Karlsson; Lingbo Sun; Zilu Ye; Yen-Hsi Chen; Katrine T Schjoldager; Catharina Steentoft; Sanae Furukawa; Barbara A Bensing; Paul M Sullam; Andrew J Thompson; James C Paulson; Christian Büll; Gosse J Adema; Ulla Mandel; Lars Hansen; Eric Paul Bennett; Ajit Varki; Sergey Y Vakhrushev; Zhang Yang; Henrik Clausen
Journal:  Mol Cell       Date:  2019-06-18       Impact factor: 17.970

3.  De novo expression of human polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6) in colon adenocarcinoma inhibits the differentiation of colonic epithelium.

Authors:  Kirstine Lavrsen; Sally Dabelsteen; Sergey Y Vakhrushev; Asha M R Levann; Amalie Dahl Haue; August Dylander; Ulla Mandel; Lars Hansen; Morten Frödin; Eric P Bennett; Hans H Wandall
Journal:  J Biol Chem       Date:  2017-11-29       Impact factor: 5.157

4.  A validated collection of mouse monoclonal antibodies to human glycosyltransferases functioning in mucin-type O-glycosylation.

Authors:  Catharina Steentoft; Zhang Yang; Shengjun Wang; Tongzhong Ju; Malene B Vester-Christensen; María F Festari; Sarah L King; Kelley Moremen; Ida S B Larsen; Christoffer K Goth; Katrine T Schjoldager; Lars Hansen; Eric P Bennett; Ulla Mandel; Yoshiki Narimatsu
Journal:  Glycobiology       Date:  2019-08-20       Impact factor: 4.313

Review 5.  Oligosaccharide Synthesis and Translational Innovation.

Authors:  Larissa Krasnova; Chi-Huey Wong
Journal:  J Am Chem Soc       Date:  2019-02-18       Impact factor: 15.419

6.  Production of homogeneous glycoprotein with multisite modifications by an engineered N-glycosyltransferase mutant.

Authors:  Qitao Song; Zhigang Wu; Yueyuan Fan; Woran Song; Peiru Zhang; Li Wang; Faxing Wang; Yangyang Xu; Peng G Wang; Jiansong Cheng
Journal:  J Biol Chem       Date:  2017-04-05       Impact factor: 5.157

7.  Glycoengineering of plants yields glycoproteins with polysialylation and other defined N-glycoforms.

Authors:  Qiang Chen
Journal:  Proc Natl Acad Sci U S A       Date:  2016-08-09       Impact factor: 11.205

8.  Proteogenomic Annotation of Chinese Hamsters Reveals Extensive Novel Translation Events and Endogenous Retroviral Elements.

Authors:  Shangzhong Li; Seong Won Cha; Kelly Heffner; Deniz Baycin Hizal; Michael A Bowen; Raghothama Chaerkady; Robert N Cole; Vijay Tejwani; Prashant Kaushik; Michael Henry; Paula Meleady; Susan T Sharfstein; Michael J Betenbaugh; Vineet Bafna; Nathan E Lewis
Journal:  J Proteome Res       Date:  2019-05-08       Impact factor: 4.466

9.  Impact of Protein Glycosylation on the Design of Viral Vaccines.

Authors:  Kathleen Schön; Bernd Lepenies; Guillaume Goyette-Desjardins
Journal:  Adv Biochem Eng Biotechnol       Date:  2021       Impact factor: 2.635

10.  Comprehensive Glycoproteomic Analysis of Chinese Hamster Ovary Cells.

Authors:  Ganglong Yang; Yingwei Hu; Shisheng Sun; Chuanzi Ouyang; Weiming Yang; Qiong Wang; Michael Betenbaugh; Hui Zhang
Journal:  Anal Chem       Date:  2018-12-03       Impact factor: 6.986
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