Literature DB >> 17113866

Lectin-resistant CHO glycosylation mutants.

Santosh Kumar Patnaik1, Pamela Stanley.   

Abstract

Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.

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Year:  2006        PMID： 17113866     DOI： 10.1016/S0076-6879(06)16011-5

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  88 in total

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