Yan-Long Tang1, Yue Zhou2, Ya-Ping Wang3, Jian-Wei Wang3, Ji-Chao Ding2. 1. Department of Radiology, Affiliated Hospital of Dali University Yunnan 671000, China. 2. Department of Histology and Embryology, Dali University Yunnan 671000, China. 3. Stem Cell and Tissue Engineering Laboratory, Department of Histology and Embryology, Chongqing Medical University Chongqing 400016, China.
Abstract
OBJECTIVE: To investigate the role of SIRT6/NF-κB signaling axis in ginsenoside Rg1-delayed hematopoietic stem/progenitor cell senescence and to provide theoretical and experimental evidence for delaying HSC/HPC senescence pathway. METHODS: After the separation and purification by immunomagnetic sorting, Sca-1+HSC/HPC was divided into: normal control group; aging group; positive control group; Rg1 delaying group and Rg1 treatment group. Senescence-associated β-galactosidase (SA-β-Gal) staining, flow cytometry analysis of cell cycle and hematopoietic progenitor cells mixed colony (CFU-Mix) culture were performed to determine the delaying or curing roles of Rg1 in Sca-1+HSC/HPC senescence. Quantitative PCR and Western blotting were used to detect the mRNA and protein expression of senescence regulatory molecules, such as SIRT6 and NF-κB. RESULTS: Compared with the aging group, the positive rate of SA-β-gal staining cells and the proportion of cells in G1 phase decreased; the number of CFU-Mix increased; mRNA and protein expression of SIRT6 increased; mRNA and protein expression of NF-κB was down-regulated in Rg1 delaying and treatment groups; the changes of the indicators in Rg1 delaying group were more significant than those in Rg1 treatment group. CONCLUSION: Rg1 may fight against Sca-1+HSC/HPC senescence induced by t-BHP through regulating SIRT6-NF-κB signaling pathway.
OBJECTIVE: To investigate the role of SIRT6/NF-κB signaling axis in ginsenosideRg1-delayed hematopoietic stem/progenitor cell senescence and to provide theoretical and experimental evidence for delaying HSC/HPC senescence pathway. METHODS: After the separation and purification by immunomagnetic sorting, Sca-1+HSC/HPC was divided into: normal control group; aging group; positive control group; Rg1 delaying group and Rg1 treatment group. Senescence-associated β-galactosidase (SA-β-Gal) staining, flow cytometry analysis of cell cycle and hematopoietic progenitor cells mixed colony (CFU-Mix) culture were performed to determine the delaying or curing roles of Rg1 in Sca-1+HSC/HPC senescence. Quantitative PCR and Western blotting were used to detect the mRNA and protein expression of senescence regulatory molecules, such as SIRT6 and NF-κB. RESULTS: Compared with the aging group, the positive rate of SA-β-gal staining cells and the proportion of cells in G1 phase decreased; the number of CFU-Mix increased; mRNA and protein expression of SIRT6 increased; mRNA and protein expression of NF-κB was down-regulated in Rg1 delaying and treatment groups; the changes of the indicators in Rg1 delaying group were more significant than those in Rg1 treatment group. CONCLUSION:Rg1 may fight against Sca-1+HSC/HPC senescence induced by t-BHP through regulating SIRT6-NF-κB signaling pathway.
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