Jing Zhang1, Wenshi Guo2, Buxian Tian2, Menghan Sun3, Hui Li4, Lina Zhou4, Xueping Liu5. 1. Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China ; Ward of Texu, Department of Gereology, First Hospital Affiliated to Liaoning Medical University Jizhou 121000, Liaoning, China. 2. Department of Neurosurgery, First Hospital Affiliated to Liaoning Medical University Jizhou 121000, Liaoning, China. 3. Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China. 4. Ward of Texu, Department of Gereology, First Hospital Affiliated to Liaoning Medical University Jizhou 121000, Liaoning, China. 5. Department of Senile Neurology, Provincial Hospital Affiliated to Shandong University Jinan 250021, Shandong, China.
Abstract
OBJECTIVE: To explored the effects of puerarin on cognitive deficits and tissue oxidative stress and the underlying mechanisms. METHODS: 6 to 8 week old male Wistar rats were adopted as experimental animals. Morris water maze (MWM) test was adopted to test the learning and memory function of rats. MDA, glutathione peroxidase and total thiol assessment was done to reflect the oxidative stress in the brain tissue. Cell Counting Kit-8 (CCK8) and flow cytometry (FCM) were performed to examine the cell viability and apoptosis rate. Reactive oxygen species (ROS) generation was determined by the 2', 7'-dichlorofluorescein diacetate (DCFH-DA) assay. qPCR and Western blot (WB) were adopted to test the molecular function mechanisms of puerarin. RESULTS: Our results indicated a protective effect of puerarin on vascular dementia. Administration of puerarin could improve the impaired learning and memory function. The levels of MDA were partially decreased by puerarin. The levels of glutathione peroxidase and total thiol were partially restored. Cell viability was improved in a dose-dependent pattern (P<0.05). Cell apoptosis rate was reduced in a dose-dependent pattern (P<0.05). Puerarin could scavenge ROS generation induced by pre-treatment of hydrogen peroxide. The results showed up-regulated levels of Nrf2, FoxO1, FoxO3 and FoxO4 (P<0.05). CONCLUSION: Puerarin is protective on the vascular dementia by reducing oxidative stress and improving learning and memory functions. On the molecular level, Nrf2, FoxO1, FoxO3 and FoxO4 were up regulated by puerarin.
OBJECTIVE: To explored the effects of puerarin on cognitive deficits and tissue oxidative stress and the underlying mechanisms. METHODS: 6 to 8 week old male Wistar rats were adopted as experimental animals. Morris water maze (MWM) test was adopted to test the learning and memory function of rats. MDA, glutathione peroxidase and total thiol assessment was done to reflect the oxidative stress in the brain tissue. Cell Counting Kit-8 (CCK8) and flow cytometry (FCM) were performed to examine the cell viability and apoptosis rate. Reactive oxygen species (ROS) generation was determined by the 2', 7'-dichlorofluorescein diacetate (DCFH-DA) assay. qPCR and Western blot (WB) were adopted to test the molecular function mechanisms of puerarin. RESULTS: Our results indicated a protective effect of puerarin on vascular dementia. Administration of puerarin could improve the impaired learning and memory function. The levels of MDA were partially decreased by puerarin. The levels of glutathione peroxidase and total thiol were partially restored. Cell viability was improved in a dose-dependent pattern (P<0.05). Cell apoptosis rate was reduced in a dose-dependent pattern (P<0.05). Puerarin could scavenge ROS generation induced by pre-treatment of hydrogen peroxide. The results showed up-regulated levels of Nrf2, FoxO1, FoxO3 and FoxO4 (P<0.05). CONCLUSION:Puerarin is protective on the vascular dementia by reducing oxidative stress and improving learning and memory functions. On the molecular level, Nrf2, FoxO1, FoxO3 and FoxO4 were up regulated by puerarin.