Literature DB >> 26183763

Development of a rapid, reliable and quantitative method--"SPOTi" for testing antifungal efficacy.

Khalida Rizi1, Sudaxshina Murdan2, Cynthia A Danquah3, Jane Faull3, Sanjib Bhakta4.   

Abstract

A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the anti-tubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and broth micro-dilution anti-fungal assays to screen novel anti-fungals.
Copyright © 2015. Published by Elsevier B.V.

Entities:  

Keywords:  Antifungals; Dermatophytes; Drug discovery; SPOTi; Screening

Mesh：

Substances：

Year:  2015        PMID： 26183763     DOI： 10.1016/j.mimet.2015.07.005

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  4 in total

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Authors:  Bullo Saifullah; Arundhati Maitra; Alina Chrzastek; Bullo Naeemullah; Sharida Fakurazi; Sanjib Bhakta; Mohd Zobir Hussein
Journal:  Molecules       Date:  2017-10-12       Impact factor: 4.411

4.  Novel Anti-Tuberculosis Nanodelivery Formulation of Ethambutol with Graphene Oxide.

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  4 in total

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