Tiande Zou1,2, Dongting He1,2, Bing Yu1,2, Jie Yu1,2, Xiangbing Mao1,2, Ping Zheng1,2, Jun He1,2, Zhiqing Huang1,2, Yan Shu1,2, Yue Liu1,2, Daiwen Chen3,4. 1. Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, 625014, Sichuan, People's Republic of China. 2. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ya'an, People's Republic of China. 3. Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an, 625014, Sichuan, People's Republic of China. dwchen@sicau.edu.cn. 4. Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ya'an, People's Republic of China. dwchen@sicau.edu.cn.
Abstract
OBJECTIVES: This study aimed to evaluate the effects of moderately increased maternal dietary energy intake during gestation on foetal skeletal muscle development and metabolism with pig as a model. METHODS: Twelve primiparous purebred Large White sows (initial body weight 135.5 ± 1.6 kg) were allocated to one of two energy intake treatments: normal-energy-intake group (Con, 30.96 MJ DE/day) as recommended by the National Research Council (NRC; 2012) and high-energy-intake group (HE, 34.15 MJ DE/day). The nutritional treatments were introduced from mating to day 90 of gestation. On day 90 of gestation, foetuses were examined by morphological, biochemical and molecular analysis of the longissimus muscle. Umbilical vein serum hormones were measured. RESULTS: Sow body weight was increased in HE group compared with Con group (P < 0.05), whereas foetal myofibre density was decreased (P < 0.05). Meanwhile, protein concentration, creatine kinase and lactate dehydrogenase activities and umbilical vein serum triiodothyronine (T3) concentration were decreased in HE foetuses (P < 0.05). Maternal HE diets decreased the mRNA abundance of muscle growth-related genes, myosin heavy-chain (MYH/MyHC) genes (MYH2 and MYH1) and insulin-like growth factor 1 and insulin growth factor-binding protein 5 (P < 0.05). Furthermore, the protein expressions of myogenic differentiation factor 1, myogenin and fast-MyHC isoforms were reduced in HE foetuses (P < 0.05). CONCLUSION: Our results suggest that moderately increased maternal dietary energy intake delays the differentiation and maturation in skeletal muscle of the foetus on day 90 of gestation.
OBJECTIVES: This study aimed to evaluate the effects of moderately increased maternal dietary energy intake during gestation on foetal skeletal muscle development and metabolism with pig as a model. METHODS: Twelve primiparous purebred Large White sows (initial body weight 135.5 ± 1.6 kg) were allocated to one of two energy intake treatments: normal-energy-intake group (Con, 30.96 MJ DE/day) as recommended by the National Research Council (NRC; 2012) and high-energy-intake group (HE, 34.15 MJ DE/day). The nutritional treatments were introduced from mating to day 90 of gestation. On day 90 of gestation, foetuses were examined by morphological, biochemical and molecular analysis of the longissimus muscle. Umbilical vein serum hormones were measured. RESULTS: Sow body weight was increased in HE group compared with Con group (P < 0.05), whereas foetal myofibre density was decreased (P < 0.05). Meanwhile, protein concentration, creatine kinase and lactate dehydrogenase activities and umbilical vein serum triiodothyronine (T3) concentration were decreased in HE foetuses (P < 0.05). Maternal HE diets decreased the mRNA abundance of muscle growth-related genes, myosin heavy-chain (MYH/MyHC) genes (MYH2 and MYH1) and insulin-like growth factor 1 and insulin growth factor-binding protein 5 (P < 0.05). Furthermore, the protein expressions of myogenic differentiation factor 1, myogenin and fast-MyHC isoforms were reduced in HE foetuses (P < 0.05). CONCLUSION: Our results suggest that moderately increased maternal dietary energy intake delays the differentiation and maturation in skeletal muscle of the foetus on day 90 of gestation.
Entities:
Keywords:
Energy intake; Foetus; Gestation; Longissimus; Myogenesis; Pig
Authors: Margaret J R Heerwagen; Melissa R Miller; Linda A Barbour; Jacob E Friedman Journal: Am J Physiol Regul Integr Comp Physiol Date: 2010-07-14 Impact factor: 3.619
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