Francesco Ciccia1, Giuliana Guggino2, Aroldo Rizzo3, Antonio Manzo4, Barbara Vitolo4, Marco Pio La Manna5, Giuseppina Giardina5, Guido Sireci5, Francesco Dieli5, Carlo Maurizio Montecucco4, Riccardo Alessandro5, Giovanni Triolo6. 1. Dipartimento Biomedico di Medicina Interna e Specialistica, Sezione di Reumatologia. 2. Dipartimento Biomedico di Medicina Interna e Specialistica, Sezione di Reumatologia, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo. 3. Azienda Ospedaliera Ospedali riuniti Villa Sofia-Cervello, Anatomia Patologica, Palermo and. 4. Rheumatology and Translational Immunology Research Laboratories, Division of Rheumatology, IRCCS Policlinico San Matteo/University of Pavia, Pavia, Italy. 5. Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo. 6. Dipartimento Biomedico di Medicina Interna e Specialistica, Sezione di Reumatologia, giovanni.triolo@unipa.it.
Abstract
OBJECTIVE: IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. METHODS: IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4(+) T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. RESULTS: IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3(+) cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. CONCLUSION: These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.
OBJECTIVE:IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. METHODS:IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4(+) T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. RESULTS:IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3(+) cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. CONCLUSION: These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.
Authors: Li-Li Pan; Juan Du; Na Gao; Hua Liao; Jin Wan; Wei-Ping Ci; Chun Yang; Tian Wang Journal: Clin Rheumatol Date: 2016-09-15 Impact factor: 2.980
Authors: Zheng Li; Xingye Li; Chao Jiang; Wenwei Qian; Gary Tse; Matthew T V Chan; William K K Wu Journal: Cell Prolif Date: 2017-11-07 Impact factor: 6.831
Authors: Simon Rauber; Markus Luber; Stefanie Weber; Lisa Maul; Alina Soare; Thomas Wohlfahrt; Neng-Yu Lin; Katharina Dietel; Aline Bozec; Martin Herrmann; Mark H Kaplan; Benno Weigmann; Mario M Zaiss; Ursula Fearon; Douglas J Veale; Juan D Cañete; Oliver Distler; Felice Rivellese; Costantino Pitzalis; Markus F Neurath; Andrew N J McKenzie; Stefan Wirtz; Georg Schett; Jörg H W Distler; Andreas Ramming Journal: Nat Med Date: 2017-07-17 Impact factor: 53.440