| Literature DB >> 26174555 |
Saiharish Raghavan1, Prasath Manogaran2, Krishna Kumari Gadepalli Narasimha3, Balasubramanian Kalpattu Kuppusami1, Palanivelu Mariyappan1, Anjana Gopalakrishnan2, Ganesh Venkatraman4.
Abstract
A number of new curcumin-quinolone hybrids were synthesised from differently substituted 3-formyl-2-quinolones and vanillin and their in vitro cytotoxicity was determined on a panel of representative cell lines (A549, MCF7, SKOV3 and H460) using MTT assay. The most potent compound 14, was analysed for its mode of action using various cell biology experiments. SKOV3 cells treated with compound 14 showed distorted cell morphology under phase contrast imaging and induction of apoptosis was confirmed by Annexin V/PE assay. Further experiments on generation of reactive oxygen species (ROS) and cell cycle analysis revealed that these hybrids induce apoptosis by ROS generation and arrest cell cycle progression in S and G2/M phase.Entities:
Keywords: Annexin-V; Cell cycle analysis; Curcumin–quinolone hybrids; MTT assay
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Year: 2015 PMID： 26174555 DOI： 10.1016/j.bmcl.2015.06.068
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823