Antonina Aydanian1, Li Tang2, Yuansha Chen2, J Glenn Morris3, Peter Olsen4, Judith A Johnson3, G Balakrish Nair5, O Colin Stine2. 1. FDA, Bethesda, Maryland, USA. 2. University of Maryland College of Medicine, Baltimore, Maryland, USA. 3. Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA. 4. Department of Computer Science, University of Maryland Baltimore County, Catonsville, MD, USA. 5. Translational Health Science and Technology Institute, Gurgaon, Haryana, India.
Abstract
BACKGROUND: In an attempt to better understand the non-O1/O139 isolates of Vibrio cholerae, a systematic study of clinical and environmental isolates collected from various geographical locations between the years 1932 and 1998 was conducted. METHODS: Ninety-nine V. cholerae isolates collected from clinical and environmental sources from various geographical regions between 1932 and 1998 were studied by sequencing seven housekeeping genes. Genetic relatedness was defined by multiple methods that allow for the observed high levels of recombination. RESULTS: Four V. cholerae subpopulations were determined. One subpopulation contained mostly environmental isolates, a second contained the cholera toxin-positive serogroup O1/O139 isolates, and the other two subpopulations were enriched for non-O1/O139 clinical isolates that were frequently clonally related to each other. CONCLUSIONS: The data suggest that many of these non-O1/O139 clinical isolates were phylogenetically related to common ancestors, even though the isolates had been collected up to 36 years apart and from different countries or continents. Published by Elsevier Ltd.
BACKGROUND: In an attempt to better understand the non-O1/O139 isolates of Vibrio cholerae, a systematic study of clinical and environmental isolates collected from various geographical locations between the years 1932 and 1998 was conducted. METHODS: Ninety-nine V. cholerae isolates collected from clinical and environmental sources from various geographical regions between 1932 and 1998 were studied by sequencing seven housekeeping genes. Genetic relatedness was defined by multiple methods that allow for the observed high levels of recombination. RESULTS: Four V. cholerae subpopulations were determined. One subpopulation contained mostly environmental isolates, a second contained the cholera toxin-positive serogroup O1/O139 isolates, and the other two subpopulations were enriched for non-O1/O139 clinical isolates that were frequently clonally related to each other. CONCLUSIONS: The data suggest that many of these non-O1/O139 clinical isolates were phylogenetically related to common ancestors, even though the isolates had been collected up to 36 years apart and from different countries or continents. Published by Elsevier Ltd.
Authors: M C Maiden; J A Bygraves; E Feil; G Morelli; J E Russell; R Urwin; Q Zhang; J Zhou; K Zurth; D A Caugant; I M Feavers; M Achtman; B G Spratt Journal: Proc Natl Acad Sci U S A Date: 1998-03-17 Impact factor: 11.205
Authors: J G Morris; T Takeda; B D Tall; G A Losonsky; S K Bhattacharya; B D Forrest; B A Kay; M Nishibuchi Journal: J Clin Invest Date: 1990-03 Impact factor: 14.808
Authors: S Chakraborty; P Garg; T Ramamurthy; M Thungapathra; J K Gautam; C Kumar; S Maiti; S Yamasaki; T Shimada; Y Takeda; A Ghosh; G B Nair Journal: J Med Microbiol Date: 2001-10 Impact factor: 2.472
Authors: C Sharma; M Thungapathra; A Ghosh; A K Mukhopadhyay; A Basu; R Mitra; I Basu; S K Bhattacharya; T Shimada; T Ramamurthy; T Takeda; S Yamasaki; Y Takeda; G B Nair Journal: J Clin Microbiol Date: 1998-03 Impact factor: 5.948
Authors: Dalong Hu; Bin Liu; Lu Feng; Peng Ding; Xi Guo; Min Wang; Boyang Cao; Peter R Reeves; Lei Wang Journal: Proc Natl Acad Sci U S A Date: 2016-11-14 Impact factor: 11.205
Authors: Jie Chen; Jian Huang; Meirong Huang; Zehui Chen; Anlin Chen; Jianru Yang; Tao Zhang; Xun Min Journal: J Int Med Res Date: 2020-06 Impact factor: 1.671