Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Cloning and sequencing of an endoglucanase (end1) gene from Butyrivibrio fibrisolvens H17c.

Literature DB >> 2615759

Cloning and sequencing of an endoglucanase (end1) gene from Butyrivibrio fibrisolvens H17c.

E Berger¹, W A Jones, D T Jones, D R Woods.

Abstract

The nucleotide sequence of a 2.8 kb DNA segment containing an endoglucanase gene (end1) from Butyrivibrio fibrisolvens H17c was determined. The B. fibrisolvens H17c gene was expressed from its own regulatory region in Escherichia coli and three putative consensus promoter sequences were identified upstream of a ribosome binding site and an ATG start codon. The complete amino acid sequence (547 residues) was deduced and homology with the Clostridium thermocellum celE gene product (EGE) was demonstrated. The endoglucanase contained a typical amino-terminal signal sequence and five repeated sequences (PDPTPVD) between amino acids 412-447. The endoglucanase showed relatively high endoglucanase activity against endoglucanase-specific substrates with beta 1-4 linkages but low activity against xylan and an exoglucanase-specific substrate, p-nitrophenyl-beta-D-cellobioside.

Entities: Chemical Species

Mesh：

Substances：
endoglucanase 1
Cellulase

Year: 1989 PMID： 2615759 DOI： 10.1007/bf00261176

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

28 in total

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

2. Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B.

Authors: O Grépinet; P Béguin
Journal: Nucleic Acids Res Date: 1986-02-25 Impact factor: 16.971

3. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors: M Dagert; S D Ehrlich
Journal: Gene Date: 1979-05 Impact factor: 3.688

4. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.

Authors: W B Wood
Journal: J Mol Biol Date: 1966-03 Impact factor: 5.469

5. Cellulolytic bacteria occurring in the rumen of sheep conditioned to low-protein teff hay.

Authors: B S Shane; L Gouws; A Kistner
Journal: J Gen Microbiol Date: 1969-03

6. Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum.

Authors: P Béguin; P Cornet; J P Aubert
Journal: J Bacteriol Date: 1985-04 Impact factor: 3.490

7. Rapid and efficient cosmid cloning.

Authors: D Ish-Horowicz; J F Burke
Journal: Nucleic Acids Res Date: 1981-07-10 Impact factor: 16.971

8. Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi.

Authors: W K Wong; B Gerhard; Z M Guo; D G Kilburn; A J Warren; R C Miller
Journal: Gene Date: 1986 Impact factor: 3.688

9. Seasonal changes in the ruminal microflora of the high-arctic Svalbard reindeer (Rangifer tarandus platyrhynchus).

Authors: C G Orpin; S D Mathiesen; Y Greenwood; A S Blix
Journal: Appl Environ Microbiol Date: 1985-07 Impact factor: 4.792

10. Structure of an endo-beta-1,4-glucanase gene from Clostridium acetobutylicum P262 showing homology with endoglucanase genes from Bacillus spp.

Authors: H Zappe; W A Jones; D T Jones; D R Woods
Journal: Appl Environ Microbiol Date: 1988-05 Impact factor: 4.792

24 in total

1. A classification of glycosyl hydrolases based on amino acid sequence similarities.

Authors: B Henrissat
Journal: Biochem J Date: 1991-12-01 Impact factor: 3.857

2. Multiple domains in endoglucanase B (CenB) from Cellulomonas fimi: functions and relatedness to domains in other polypeptides.

Authors: A Meinke; N R Gilkes; D G Kilburn; R C Miller; R A Warren
Journal: J Bacteriol Date: 1991-11 Impact factor: 3.490

Review 3. Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Authors: N R Gilkes; B Henrissat; D G Kilburn; R C Miller; R A Warren
Journal: Microbiol Rev Date: 1991-06

4. Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabinofuranosidase activities.

Authors: E A Utt; C K Eddy; K F Keshav; L O Ingram
Journal: Appl Environ Microbiol Date: 1991-04 Impact factor: 4.792

5. DNA sequence of a Fibrobacter succinogenes mixed-linkage beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase) gene.

Authors: R M Teather; J D Erfle
Journal: J Bacteriol Date: 1990-07 Impact factor: 3.490

6. Conjugal transfer of Tn916, Tn916 delta E, and pAM beta 1 from Enterococcus faecalis to Butyrivibrio fibrisolvens strains.

Authors: R B Hespell; T R Whitehead
Journal: Appl Environ Microbiol Date: 1991-09 Impact factor: 4.792

7. Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase.

Authors: L Zhou; G P Xue; C G Orpin; G W Black; H J Gilbert; G P Hazlewood
Journal: Biochem J Date: 1994-01-15 Impact factor: 3.857

8. Expression of the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene together with the Erwinia pectate lyase and polygalacturonase genes in Saccharomyces cerevisiae.

Authors: P van Rensburg; W H van Zyl; I S Pretorius
Journal: Curr Genet Date: 1994-12 Impact factor: 3.886

9. Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity.

Authors: E Rumbak; D E Rawlings; G G Lindsey; D R Woods
Journal: J Bacteriol Date: 1991-11 Impact factor: 3.490

10. Characterization of hybrid proteins consisting of the catalytic domains of Clostridium and Ruminococcus endoglucanases, fused to Pseudomonas non-catalytic cellulose-binding domains.

Authors: D M Poole; A J Durrant; G P Hazlewood; H J Gilbert
Journal: Biochem J Date: 1991-11-01 Impact factor: 3.857