Jennifer S McDaniel1, Marcello Pilia1, Vivek Raut2, Jeffrey Ledford1, Stefanie M Shiels1, Joseph C Wenke1, Brian Barnes2, Christopher R Rathbone3. 1. Extremity Trauma and Regenerative Medicine, United States Army Institute of Surgical Research, 3698 Chambers Pass BLDG 3611, JBSA Fort Sam Houston, TX, 78234, USA. 2. Arteriocyte Medical Systems, Hopkinton, MA, USA. 3. Extremity Trauma and Regenerative Medicine, United States Army Institute of Surgical Research, 3698 Chambers Pass BLDG 3611, JBSA Fort Sam Houston, TX, 78234, USA. chrisrathbone29@gmail.com.
Abstract
PURPOSE: This study was designed to identify strategies for treating bone defects that can be completed on the day of surgery. METHODS: Forty New Zealand white rabbits with unilateral rabbit radius segmental defects (15 mm) were treated with commercially available scaffolds containing either demineralised bone matrix (DBM) or a collagen/beta-tricalcium phosphate composite (Col:β-TCP); each scaffold was combined with either bone marrow aspirate (BMA) or concentrated BMA (cBMA). Bone regeneration was assessed through radiographic and histological analyses. RESULTS: The concentration of nucleated cells, colony-forming unit-fibroblasts and platelets were increased and haematocrit concentration decreased in cBMA as compared to BMA (p < 0.05). Radiographic analyses of bone formation and defect bridging demonstrated significantly greater bone regeneration in the defects treated with DBM grafts as compared to Col:β-TCP grafts. The healing of bones treated with Col:β-TCP was improved when augmented with cBMA. CONCLUSIONS: Scaffolds containing either DBM or Col:β-TCP with BMA or cBMA are effective same-day strategies available to clinicians for the treatment of bone defects; the latter scaffold may be more effective if combined with cBMA.
PURPOSE: This study was designed to identify strategies for treating bone defects that can be completed on the day of surgery. METHODS: Forty New Zealand white rabbits with unilateral rabbit radius segmental defects (15 mm) were treated with commercially available scaffolds containing either demineralised bone matrix (DBM) or a collagen/beta-tricalcium phosphate composite (Col:β-TCP); each scaffold was combined with either bone marrow aspirate (BMA) or concentrated BMA (cBMA). Bone regeneration was assessed through radiographic and histological analyses. RESULTS: The concentration of nucleated cells, colony-forming unit-fibroblasts and platelets were increased and haematocrit concentration decreased in cBMA as compared to BMA (p < 0.05). Radiographic analyses of bone formation and defect bridging demonstrated significantly greater bone regeneration in the defects treated with DBM grafts as compared to Col:β-TCP grafts. The healing of bones treated with Col:β-TCP was improved when augmented with cBMA. CONCLUSIONS: Scaffolds containing either DBM or Col:β-TCP with BMA or cBMA are effective same-day strategies available to clinicians for the treatment of bone defects; the latter scaffold may be more effective if combined with cBMA.
Entities:
Keywords:
Bone marrow aspirate; Demineralised bone matrix; Rabbit; Segmental defect; Tricalcium phosphate
Authors: William R Walsh; Frank Vizesi; Dean Michael; Jason Auld; Andy Langdown; Rema Oliver; Yan Yu; Hiroyuki Irie; Warwick Bruce Journal: Biomaterials Date: 2008-01 Impact factor: 12.479