Literature DB >> 26155475

Rapid Detection of Mycobacterium tuberculosis in Sputum Samples by Cepheid Xpert Assay: A Clinical Study.

Deepak Arora1, Neerja Jindal2, Renu Bansal3, Shilpa Arora4.   

Abstract

BACKGROUND AND
OBJECTIVE: Tuberculosis (TB) is one of the commonest opportunistic infection and the leading cause of death in HIV patients in developing countries. HIV infection is a well recognised risk factor for both activation of initial infection and reactivation of latent infection. This study was done to find out the co-prevalence and the trend of HIV infection among tuberculosis patients and to determine the prevalence of MDR Tuberculosis in HIV positive patients using Xpert MTB/RIF assay (Cepheid, Sunnyvale, CA, United States).
MATERIALS AND METHODS: The sputum samples are received from five districts of Punjab. Sputum samples of the patients with symptoms suggestive of pulmonary tuberculosis including both new cases and on treatment are received from the various district and civil hospitals of five districts. Sputum specimen was processed for TB detection by Cartridge Based Nucleic Acid Amplification testing (CB-NAAT) using Xpert MTB/RiF assay technology.
RESULTS: The study period is from October 2013 to September 2014. A total of 907 patients with symptoms suggestive of pulmonary tuberculosis including both new cases and on treatment. Out of these patients 733 were reported MTB detected. Out of these 907 patients 29 were reported HIV positive and out of 733 patients 19(2.5%) were reported positive for (HIV +TUBERCULOSIS). Of these 19 cases 16(84.21%) cases were sensitive to rifampicin (RIF) and 3(15.78%) cases were showing resistance to rifampicin (RIF) Drug.
CONCLUSION: Co-existence of HIV and tuberculosis is high and there is high Prevalence of MDR tuberculosis in HIV patients.

Entities:  

Keywords:  Cartridge based nucleic acid amplification testing Human immunodeficiency virus (HIV); Co- infection; Pulmonary; Rifampicin; Tuberculosis

Year:  2015        PMID: 26155475      PMCID: PMC4484067          DOI: 10.7860/JCDR/2015/11352.5935

Source DB:  PubMed          Journal:  J Clin Diagn Res        ISSN: 0973-709X


  13 in total

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Authors:  Karin Weyer; Fuad Mirzayev; Giovanni Battista Migliori; Wayne Van Gemert; Lia D'Ambrosio; Matteo Zignol; Katherine Floyd; Rosella Centis; Daniela M Cirillo; Enrico Tortoli; Chris Gilpin; Jean de Dieu Iragena; Dennis Falzon; Mario Raviglione
Journal:  Eur Respir J       Date:  2012-11-22       Impact factor: 16.671

2.  Transforming TB diagnosis: can patients and control programs afford to wait?

Authors:  Elizabeth A Talbot; Jean W Pape; Lakshmi Sundaram; Catharina C Boehme; Mark D Perkins
Journal:  Am J Trop Med Hyg       Date:  2012-08       Impact factor: 2.345

3.  False-positive Xpert® MTB/RIF assays in previously treated patients: need for caution in interpreting results.

Authors:  T H Boyles; J Hughes; V Cox; R Burton; G Meintjes; M Mendelson
Journal:  Int J Tuberc Lung Dis       Date:  2014-07       Impact factor: 2.373

Review 4.  Xpert® MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults.

Authors:  Karen R Steingart; Ian Schiller; David J Horne; Madhukar Pai; Catharina C Boehme; Nandini Dendukuri
Journal:  Cochrane Database Syst Rev       Date:  2014-01-21

5.  Catching the missing million: experiences in enhancing TB & DR-TB detection by providing upfront Xpert MTB/RIF testing for people living with HIV in India.

Authors:  Neeraj Raizada; Kuldeep Singh Sachdeva; Achuthan Sreenivas; Shubhangi Kulsange; Radhey Shyam Gupta; Rahul Thakur; Puneet Dewan; Catharina Boehme; Chinnambedu Nainarappan Paramsivan
Journal:  PLoS One       Date:  2015-02-06       Impact factor: 3.240

6.  Promoting affordable and quality tuberculosis testing in India.

Authors:  Madhukar Pai
Journal:  J Lab Physicians       Date:  2013-01

7.  Meta-analysis to compare the accuracy of GeneXpert, MODS and the WHO 2007 algorithm for diagnosis of smear-negative pulmonary tuberculosis.

Authors:  Simon Walusimbi; Freddie Bwanga; Ayesha De Costa; Melles Haile; Moses Joloba; Sven Hoffner
Journal:  BMC Infect Dis       Date:  2013-10-30       Impact factor: 3.090

8.  Enhancing TB case detection: experience in offering upfront Xpert MTB/RIF testing to pediatric presumptive TB and DR TB cases for early rapid diagnosis of drug sensitive and drug resistant TB.

Authors:  Neeraj Raizada; Kuldeep Singh Sachdeva; Sreenivas Achuthan Nair; Shubhangi Kulsange; Radhey Shayam Gupta; Rahul Thakur; Malik Parmar; Christen Gray; Ranjani Ramachandran; Bhavin Vadera; Shobha Ekka; Shikha Dhawan; Ameet Babre; Mayank Ghedia; Umesh Alavadi; Puneet Dewan; Mini Khetrapal; Ashwini Khanna; Catharina Boehme; Chinnambedu Nainarappan Paramsivan
Journal:  PLoS One       Date:  2014-08-20       Impact factor: 3.240

9.  Effects of introducing Xpert MTB/RIF test on multi-drug resistant tuberculosis diagnosis in KwaZulu-Natal South Africa.

Authors:  Nomonde R Dlamini-Mvelase; Lise Werner; Rogerio Phili; Lindiwe P Cele; Koleka P Mlisana
Journal:  BMC Infect Dis       Date:  2014-08-16       Impact factor: 3.090

10.  Prevalence of multidrug resistance in Mycobacterium tuberculosis isolates from HIV seropositive and seronegative patients with pulmonary tuberculosis in north India.

Authors:  Sunil Sethi; Abhishek Mewara; Sunil Kumar Dhatwalia; Harpal Singh; Rakesh Yadav; Khushwinder Singh; Dheeraj Gupta; Ajay Wanchu; Meera Sharma
Journal:  BMC Infect Dis       Date:  2013-03-15       Impact factor: 3.090

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Review 1.  Guidelines for Preoperative Testing for Neurosurgery in Coronavirus Disease 2019 (COVID-19) Era: Indian Viewpoint Amidst Global Practice.

Authors:  Nitish Agarwal; Amol Raheja; Ashish Suri
Journal:  World Neurosurg       Date:  2020-10-24       Impact factor: 2.104

2.  GeneXpert Technology for the diagnosis of HIV-associated tuberculosis: Is scale-up worth it?

Authors:  Muhammad Saeed; Shahida Hussain; Saba Riaz; Farhan Rasheed; Maqsood Ahmad; Shagufta Iram; Mizna Arif; Tariq Hamid Rahmani; Ambereen Imran Anwar
Journal:  Open Life Sci       Date:  2020-06-30       Impact factor: 0.938

Review 3.  Effectiveness of GeneXpert® in the diagnosis of tuberculosis in people living with HIV/AIDS.

Authors:  Mariana Gaspar Botelho Funari de Faria; Rubia Laine de Paula Andrade; Ana Julia Gonçalves Camillo; Karina Fonseca de Souza Leite; Nanci Michele Saita; Valdes Roberto Bollela; Carlos Eduardo Menezes de Rezende; Aline Aparecida Monroe
Journal:  Rev Saude Publica       Date:  2021-12-17       Impact factor: 2.106

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