Literature DB >> 26151408

Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae.

Kazumasa Shiraiwa1, Yohsuke Ogawa1, Masahiro Eguchi1, Hirokazu Hikono1, Masahiro Kusumoto1, Yoshihiro Shimoji2.   

Abstract

The differentiation of vaccine from non-vaccine isolates is important for disease control. Based on single nucleotide polymorphisms identified by comparison of the genomes of the Koganei 65-0.15 vaccine strain and a reference strain of Erysipelothrix rhusiopathiae, we developed a PCR assay that can differentiate the vaccine strain from field isolates.
Copyright © 2015 Elsevier B.V. All rights reserved.

Keywords:  Erysipelothrix rhusiopathiae; Genome sequencing; SNP-based PCR; Strain-specific detection; Vaccine strain

Mesh：

Substances：

Year:  2015        PMID： 26151408     DOI： 10.1016/j.mimet.2015.07.001

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  8 in total

1.  Whole genome analysis of more than 10 000 SARS-CoV-2 virus unveils global genetic diversity and target region of NSP6.

Authors:  Indrajit Saha; Nimisha Ghosh; Ayan Pradhan; Nikhil Sharma; Debasree Maity; Kaushik Mitra
Journal:  Brief Bioinform       Date:  2021-03-22       Impact factor: 11.622

2.  Clonal Lineages of Erysipelothrix rhusiopathiae Responsible for Acute Swine Erysipelas in Japan Identified by Using Genome-Wide Single-Nucleotide Polymorphism Analysis.

Authors:  Yohsuke Ogawa; Kazumasa Shiraiwa; Yoshitoshi Ogura; Tadasuke Ooka; Sayaka Nishikawa; Masahiro Eguchi; Tetsuya Hayashi; Yoshihiro Shimoji
Journal:  Appl Environ Microbiol       Date:  2017-05-17       Impact factor: 4.792

3.  Genome-Wide Identification of Virulence Genes in Erysipelothrix rhusiopathiae: Use of a Mutant Deficient in a tagF Homolog as a Safe Oral Vaccine against Swine Erysipelas.

Authors:  Yoshihiro Shimoji; Yohsuke Ogawa; Manae Tsukio; Kazumasa Shiraiwa; Sayaka Nishikawa; Masahiro Eguchi
Journal:  Infect Immun       Date:  2019-11-18       Impact factor: 3.441

4.  Identification of the Chromosomal Region Essential for Serovar-Specific Antigen and Virulence of Serovar 1 and 2 Strains of Erysipelothrix rhusiopathiae.

Authors:  Yohsuke Ogawa; Kazumasa Shiraiwa; Sayaka Nishikawa; Masahiro Eguchi; Yoshihiro Shimoji
Journal:  Infect Immun       Date:  2018-08-22       Impact factor: 3.441

5.  Pathogenic characterization of Erysipelothrix rhusiopathiae Met-203 type SpaA strains from chronic and subacute swine erysipelas in Japan.

Authors:  Mariko Uchiyama; Yohko Shimazaki; Yukari Isshiki; Akemi Kojima; Fumiya Hirano; Kinya Yamamoto; Mayumi Kijima; Hidetaka Nagai
Journal:  J Vet Med Sci       Date:  2016-10-21       Impact factor: 1.267

6.  Multiplex PCR assay for the simultaneous detection and differentiation of clonal lineages of Erysipelothrix rhusiopathiae serovar 1a strains currently circulating in Japan.

Authors:  Kazumasa Shiraiwa; Yohsuke Ogawa; Sayaka Nishikawa; Masahiro Eguchi; Yoshihiro Shimoji
Journal:  J Vet Med Sci       Date:  2017-06-22       Impact factor: 1.267

7.  Single nucleotide polymorphism genotyping of Erysipelothrix rhusiopathiae isolates from pigs affected with chronic erysipelas in Japan.

Authors:  Kazumasa Shiraiwa; Yohsuke Ogawa; Sayaka Nishikawa; Masahiro Kusumoto; Masahiro Eguchi; Yoshihiro Shimoji
Journal:  J Vet Med Sci       Date:  2017-03-02       Impact factor: 1.267

8.  Development of Single Nucleotide Polymorphism (SNP)-Based Triplex PCR Marker for Serotype-specific Escherichia coli Detection.

Authors:  Md-Mafizur Rahman; Sang-Jin Lim; Yung-Chul Park
Journal:  Pathogens       Date:  2022-01-19
  8 in total

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