Mariza Abreu Miranda1, Marivane Lemos2, Kamila Alves Cowart3, Douglas Rodenburg4, James D McChesney5, Mohamed M Radwan6, Niege Araçari Jacometti Cardoso Furtado7, Jairo Kenupp Bastos8. 1. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, s/n, 14040-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: marizamiranda2003@yahoo.com.br. 2. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, s/n, 14040-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: marivanelemos@gmail.com. 3. Ironstone Separations, Inc., 851B Highway 30 East, Oxford, MS 38655, USA. Electronic address: kamilla_07@yahoo.com. 4. Ironstone Separations, Inc., 851B Highway 30 East, Oxford, MS 38655, USA. Electronic address: douglasrodenburg@yahoo.com. 5. Ironstone Separations, Inc., 851B Highway 30 East, Oxford, MS 38655, USA. Electronic address: jdmcchesney@yahoo.com. 6. National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA. Electronic address: mradwan@olemiss.edu. 7. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, s/n, 14040-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: niege@fcfrp.usp.br. 8. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, s/n, 14040-903 Ribeirão Preto, São Paulo, Brazil. Electronic address: jkbastos@fcfrp.usp.br.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Solanum cernuum Vell. (Solanaceae) is a Brazilian medicinal plant, traditionally known as "panaceia". Its folk name is probably due to its wide range of applications in traditional medicine including the treatment of ulcers. AIM OF THE STUDY: To evaluate the gastroprotective activities of the hydroethanolic extract (ESC) of S. cernuum and its major isolated compounds using in vivo gastric ulcer models. MATERIAL AND METHODS: The ESC extract was obtained by maceration followed by percolation of the dried and powdered leaves of S. cernuum in ethanol:water (7:3). The major compounds in the extract were isolated by applying various preparative chromatographic techniques. The gastroprotective activity was evaluated in mice using different gastric ulcer-induced models. The anti-Helicobacter pylori activity was performed using the agar-well diffusion and broth microdilution methods. RESULTS: The ESC extract showed gastroprotective effects in the assay of acute gastric ulcer-induced by HCl/EtOH, nonsteroidal anti-inflammatory drug, and acetic acid-induced chronic ulcer protocols. The results also demonstrated that the gastroprotection induced by ESC extract is related to the activity of nitric oxide and endogenous sulfhydryls, which are important gastroprotective factors. The ESC extract and the alkaloid cernumidine did not show activity against H. pylori in the concentrations tested. CONCLUSIONS: The present study showed that the crude extract of S. cernuum possessed gastroprotective activity which corroborating the traditional use of this plant for the treatment of gastric ulcers. The isolated flavonoids, quercitrin and afzelin as well as the phenylpropanoid, isoferulic acid are suggested to be the compounds responsible for the gastroprotective activity of S. cernuum extract.
ETHNOPHARMACOLOGICAL RELEVANCE: Solanum cernuum Vell. (Solanaceae) is a Brazilian medicinal plant, traditionally known as "panaceia". Its folk name is probably due to its wide range of applications in traditional medicine including the treatment of ulcers. AIM OF THE STUDY: To evaluate the gastroprotective activities of the hydroethanolic extract (ESC) of S. cernuum and its major isolated compounds using in vivo gastric ulcer models. MATERIAL AND METHODS: The ESC extract was obtained by maceration followed by percolation of the dried and powdered leaves of S. cernuum in ethanol:water (7:3). The major compounds in the extract were isolated by applying various preparative chromatographic techniques. The gastroprotective activity was evaluated in mice using different gastric ulcer-induced models. The anti-Helicobacter pylori activity was performed using the agar-well diffusion and broth microdilution methods. RESULTS: The ESC extract showed gastroprotective effects in the assay of acute gastric ulcer-induced by HCl/EtOH, nonsteroidal anti-inflammatory drug, and acetic acid-induced chronic ulcer protocols. The results also demonstrated that the gastroprotection induced by ESC extract is related to the activity of nitric oxide and endogenous sulfhydryls, which are important gastroprotective factors. The ESC extract and the alkaloid cernumidine did not show activity against H. pylori in the concentrations tested. CONCLUSIONS: The present study showed that the crude extract of S. cernuum possessed gastroprotective activity which corroborating the traditional use of this plant for the treatment of gastric ulcers. The isolated flavonoids, quercitrin and afzelin as well as the phenylpropanoid, isoferulic acid are suggested to be the compounds responsible for the gastroprotective activity of S. cernuum extract.
Authors: Mohamed Yousif Ibrahim; Najihah Mohd Hashim; Summaya M Dhiyaaldeen; Mazen M Jamil Al-Obaidi; Rashd M El-Ferjani; Hoyam Adam; Bassam Alkotaini; Rami Al Batran; Hapipah Mohd Ali Journal: Sci Rep Date: 2016-05-27 Impact factor: 4.379