Literature DB >> 26135892

Extended-Spectrum β-Lactamase (ESBL)-Producing Klebsiella pneumoniae in Bulk Tank Milk from Dairy Farms in Indonesia.

Mirnawati Sudarwanto1, Ömer Akineden2, Sabrina Odenthal2, Madeleine Gross2, Ewald Usleber2.   

Abstract

Bulk tank milk from 80 dairy farms located in the West Java Region of Indonesia was analyzed for the presence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. Isolates from seven dairy farms were ESBL positive, and all were identified as Klebsiella pneumoniae. The isolates showed ESBL-characteristic antibiotic resistance patterns. Further analysis revealed that all K. pneumoniae isolates harbored the blaSHV gene, and two isolates were additionally positive for the blaTEM-1 and blaCTX-M-15 genes. Isolates from different farms were clonally diverse according to macrorestriction analysis. The results indicate that the relatively high frequency of ESBL-producing K. pneumoniae in bulk tank milk implies the risk that milk is both a source of local exposure and a vector contributing to the supraregional spread of antibiotic-resistant bacteria by trade.

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Year:  2015        PMID: 26135892     DOI: 10.1089/fpd.2014.1895

Source DB:  PubMed          Journal:  Foodborne Pathog Dis        ISSN: 1535-3141            Impact factor:   3.171


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