| Literature DB >> 26122311 |
Xiaomu Liu1,2, Wei You1,2, Haijian Cheng1,2, Qingfeng Zhang3, Enliang Song1,2, Fachun Wan1,2, Hong Han1,2, Guifen Liu4,5.
Abstract
Mevalonic acid (MVA) is a key material in the synthesis of cholesterol; indeed, intracellular cholesterol synthesis is also called the mevalonic acid pathway. 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is an essential enzyme in cholesterol biosynthesis. This study suggests that MVA may play an important role in the differentiation of bovine adipose tissue in vivo. We investigated differential mRNA expression in bovine intramuscular preadipocytes (BIPs) and bovine subcutaneous preadipocytes (BSPs) by culturing cells from the longissimus dorsi muscle and subcutaneous fat tissues of Luxi yellow cattle. The morphology of lipid accumulation of bovine preadipocytes was detected by Oil Red O staining, and total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), and high-density lipoprotein cholesterol (HDLC) levels were measured. Temporospatial expression of HMGR was investigated by real-time quantitative polymerase chain reaction (PCR). The TC, LDLC, and HDLC content did not significantly differ over time but increased slowly with increasing MVA concentration. HMGR expression increased over time and with increasing concentrations of MVA. MVA increased adipose cell proliferation in a dose-dependent and time-dependent manner. MVA stimulated HMGR expression in two cell types and its influence on adipocyte differentiation.Entities:
Keywords: 3-Hydroxy-3-methylglutaryl-CoA reductase; Bovine adipocytes; Cholesterol; Meat quality; Mevalonic acid
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Year: 2015 PMID： 26122311 DOI： 10.1007/s13353-015-0300-y
Source DB: PubMed Journal: J Appl Genet ISSN： 1234-1983 Impact factor: 3.240