Literature DB >> 26121321

Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning.

Ayca Yalcin Ozkumur1, Brittany A Goods2, J Christopher Love3,4,5.   

Abstract

Living-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  cell patterning; human immunodeficiency virus (HIV); living-cell arrays; neutralization

Mesh：

Substances：

Year:  2015        PMID： 26121321      PMCID： PMC4754792          DOI： 10.1002/smll.201500674

Source DB:  PubMed          Journal:  Small        ISSN： 1613-6810            Impact factor:   13.281


  28 in total

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Authors:  Stuart G Baker
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2.  Sensitivity of HIV-1 to entry inhibitors correlates with envelope/coreceptor affinity, receptor density, and fusion kinetics.

Authors:  Jacqueline D Reeves; Stephen A Gallo; Navid Ahmad; John L Miamidian; Phoebe E Harvey; Matthew Sharron; Stefan Pohlmann; Jeffrey N Sfakianos; Cynthia A Derdeyn; Robert Blumenthal; Eric Hunter; Robert W Doms
Journal:  Proc Natl Acad Sci U S A       Date:  2002-11-20       Impact factor: 11.205

3.  Controlling mammalian cell interactions on patterned polyelectrolyte multilayer surfaces.

Authors:  Michael C Berg; Sung Yun Yang; Paula T Hammond; Michael F Rubner
Journal:  Langmuir       Date:  2004-02-17       Impact factor: 3.882

4.  A microengraving method for rapid selection of single cells producing antigen-specific antibodies.

Authors:  J Christopher Love; Jehnna L Ronan; Gijsbert M Grotenbreg; Annemarthe G van der Veen; Hidde L Ploegh
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5.  Comparative study and improvement of current cell micro-patterning techniques.

Authors:  Jenny Fink; Manuel Théry; Ammar Azioune; Raphael Dupont; François Chatelain; Michel Bornens; Matthieu Piel
Journal:  Lab Chip       Date:  2007-04-30       Impact factor: 6.799

6.  Fine mapping of the interaction of neutralizing and nonneutralizing monoclonal antibodies with the CD4 binding site of human immunodeficiency virus type 1 gp120.

Authors:  Ralph Pantophlet; Erica Ollmann Saphire; Pascal Poignard; Paul W H I Parren; Ian A Wilson; Dennis R Burton
Journal:  J Virol       Date:  2003-01       Impact factor: 5.103

7.  Kinetic factors control efficiencies of cell entry, efficacies of entry inhibitors, and mechanisms of adaptation of human immunodeficiency virus.

Authors:  Emily J Platt; James P Durnin; David Kabat
Journal:  J Virol       Date:  2005-04       Impact factor: 5.103

8.  An extracellular matrix microarray for probing cellular differentiation.

Authors:  Christopher J Flaim; Shu Chien; Sangeeta N Bhatia
Journal:  Nat Methods       Date:  2005-01-21       Impact factor: 28.547

Review 9.  High-throughput cellular microarray platforms: applications in drug discovery, toxicology and stem cell research.

Authors:  Tiago G Fernandes; Maria Margarida Diogo; Douglas S Clark; Jonathan S Dordick; Joaquim M S Cabral
Journal:  Trends Biotechnol       Date:  2009-05-03       Impact factor: 19.536

10.  Screening individual hybridomas by microengraving to discover monoclonal antibodies.

Authors:  Adebola O Ogunniyi; Craig M Story; Eliseo Papa; Eduardo Guillen; J Christopher Love
Journal:  Nat Protoc       Date:  2009       Impact factor: 13.491
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2.  Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.

Authors:  Ada Hang-Heng Wong; Haoran Li; Yanwei Jia; Pui-In Mak; Rui Paulo da Silva Martins; Yan Liu; Chi Man Vong; Hang Cheong Wong; Pak Kin Wong; Haitao Wang; Heng Sun; Chu-Xia Deng
Journal:  Sci Rep       Date:  2017-08-22       Impact factor: 4.379

3.  Toward point-of-care assessment of patient response: a portable tool for rapidly assessing cancer drug efficacy using multifrequency impedance cytometry and supervised machine learning.

Authors:  Karan Ahuja; Gulam M Rather; Zhongtian Lin; Jianye Sui; Pengfei Xie; Tuan Le; Joseph R Bertino; Mehdi Javanmard
Journal:  Microsyst Nanoeng       Date:  2019-07-15       Impact factor: 7.127
  3 in total

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