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Literature DB >> 26119740

Piwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis.

Jacob Gonzalez¹, Hongying Qi¹, Na Liu¹, Haifan Lin².

Abstract

The Piwi-piRNA pathway is well known for its germline function, yet its somatic role remains elusive. We show here that Piwi is required autonomously not only for germline stem cell (GSC) but also for somatic cyst stem cell (CySC) maintenance in the Drosophila testis. Reducing Piwi activity in the testis caused defects in CySC differentiation. Accompanying this, GSC daughters expanded beyond the vicinity of the hub but failed to differentiate further. Moreover, Piwi deficient in nuclear localization caused similar defects in somatic and germ cell differentiation, which was rescued by somatic Piwi expression. To explore the underlying molecular mechanism, we identified Piwi-bound piRNAs that uniquely map to a gene key for gonadal development, Fasciclin 3, and demonstrate that Piwi regulates its expression in somatic cyst cells. Our work reveals the cell-autonomous function of Piwi in both somatic and germline stem cell types, with somatic function possibly via its epigenetic mechanism.

Entities: Chemical Disease Gene Mutation Species

Mesh：

Substances：

Year: 2015 PMID： 26119740 PMCID： PMC4497877 DOI： 10.1016/j.celrep.2015.06.004

Source DB: PubMed Journal: Cell Rep Impact factor: 9.423

40 in total

1. A broadly conserved pathway generates 3'UTR-directed primary piRNAs.

Authors: Nicolas Robine; Nelson C Lau; Sudha Balla; Zhigang Jin; Katsutomo Okamura; Satomi Kuramochi-Miyagawa; Michael D Blower; Eric C Lai
Journal: Curr Biol Date: 2009-12-29 Impact factor: 10.834

2. Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo.

Authors: Christel Rouget; Catherine Papin; Anthony Boureux; Anne-Cécile Meunier; Bénédicte Franco; Nicolas Robine; Eric C Lai; Alain Pelisson; Martine Simonelig
Journal: Nature Date: 2010-10-17 Impact factor: 49.962

3. An epigenetic activation role of Piwi and a Piwi-associated piRNA in Drosophila melanogaster.

Authors: Hang Yin; Haifan Lin
Journal: Nature Date: 2007-10-21 Impact factor: 49.962

4. Zfh-1 controls somatic stem cell self-renewal in the Drosophila testis and nonautonomously influences germline stem cell self-renewal.

Authors: Judith L Leatherman; Stephen Dinardo
Journal: Cell Stem Cell Date: 2008-07-03 Impact factor: 24.633

5. A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila.

Authors: Kuniaki Saito; Sachi Inagaki; Toutai Mituyama; Yoshinori Kawamura; Yukiteru Ono; Eri Sakota; Hazuki Kotani; Kiyoshi Asai; Haruhiko Siomi; Mikiko C Siomi
Journal: Nature Date: 2009-10-07 Impact factor: 49.962

Review 6. Beyond transposons: the epigenetic and somatic functions of the Piwi-piRNA mechanism.

Authors: Jamy C Peng; Haifan Lin
Journal: Curr Opin Cell Biol Date: 2013-03-04 Impact factor: 8.382

7. piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells.

Authors: D N Cox; A Chao; H Lin
Journal: Development Date: 2000-02 Impact factor: 6.868

8. Piwi is required in multiple cell types to control germline stem cell lineage development in the Drosophila ovary.

Authors: Xing Ma; Su Wang; Trieu Do; Xiaoqing Song; Mayu Inaba; Yoshiya Nishimoto; Lu-ping Liu; Yuan Gao; Ying Mao; Hui Li; William McDowell; Jungeun Park; Kate Malanowski; Allison Peak; Anoja Perera; Hua Li; Karin Gaudenz; Jeff Haug; Yukiko Yamashita; Haifan Lin; Jian-quan Ni; Ting Xie
Journal: PLoS One Date: 2014-03-21 Impact factor: 3.240

Review 9. Stem cells and niches: mechanisms that promote stem cell maintenance throughout life.

Authors: Sean J Morrison; Allan C Spradling
Journal: Cell Date: 2008-02-22 Impact factor: 41.582

10. Retrotransposons and pseudogenes regulate mRNAs and lncRNAs via the piRNA pathway in the germline.

Authors: Toshiaki Watanabe; Ee-chun Cheng; Mei Zhong; Haifan Lin
Journal: Genome Res Date: 2014-12-05 Impact factor: 9.043

31 in total

1. Possible role of HIWI2 in modulating tight junction proteins in retinal pigment epithelial cells through Akt signaling pathway.

Authors: Suganya Sivagurunathan; Karthikka Palanisamy; Jayamuruga Pandian Arunachalam; Subbulakshmi Chidambaram
Journal: Mol Cell Biochem Date: 2016-12-26 Impact factor: 3.396

Review 2. Protecting and Diversifying the Germline.

Authors: Ryan J Gleason; Amit Anand; Toshie Kai; Xin Chen
Journal: Genetics Date: 2018-02 Impact factor: 4.562

3. Expression of Piwi protein MIWI2 defines a distinct population of multiciliated cells.

Authors: Gregory A Wasserman; Aleksander D Szymaniak; Anne C Hinds; Kazuko Yamamoto; Hirofumi Kamata; Nicole Ms Smith; Kristie L Hilliard; Claudia Carrieri; Adam T Labadorf; Lee J Quinton; Xingbin Ai; Xaralabos Varelas; Felicia Chen; Joseph P Mizgerd; Alan Fine; Dónal O'Carroll; Matthew R Jones
Journal: J Clin Invest Date: 2017-09-18 Impact factor: 14.808

Review 4. Regulation of Drosophila germline stem cells.

Authors: Elizabeth W Kahney; Jonathan C Snedeker; Xin Chen
Journal: Curr Opin Cell Biol Date: 2019-04-20 Impact factor: 8.382

5. piRNAs Interact with Cold-Shock Domain-Containing RNA Binding Proteins and Regulate Neuronal Gene Expression During Differentiation.

Authors: Charannya Sozheesvari Subhramanyam; Qiong Cao; Cheng Wang; Zealyn Shi-Lin Heng; Zhihong Zhou; Qidong Hu
Journal: Mol Neurobiol Date: 2022-01-04 Impact factor: 5.590