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Literature DB >> 2611914

A petite positive strain of Kluyveromyces lactis has a 300 kb deletion in the rDNA cluster.

Abstract

By employing Pulsed Field Gel Electrophoresis we have shown that the chromosomal DNA pattern of a petite-positive strain of Kluyveromyces lactis, designated KF4, differs from the karyotype of the reference strain. Digestion with SfiI and hybridization with an rDNA probe, demonstrate that a novel chromosomal band in KF4 results from a deletion of 35-40 rDNA cistrons from the rDNA cluster. The significance of this finding to possible alterations in cell physiology and the ability to generate "petite" mutants is discussed.

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Year: 1989 PMID： 2611914 DOI： 10.1007/bf00340722

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

10 in total

Review 1. The genome of bakers yeast-the benchmark for a eukaryotic cell.

Authors: G D Clark-Walker
Journal: Yeast Date: 1989-04 Impact factor: 3.239

Review 2. Pulsed field gel electrophoresis.

Authors: E Lai; B W Birren; S M Clark; M I Simon; L Hood
Journal: Biotechniques Date: 1989-01 Impact factor: 1.993

3. KGB: a single buffer for all restriction endonucleases.

Authors: M McClelland; J Hanish; M Nelson; Y Patel
Journal: Nucleic Acids Res Date: 1988-01-11 Impact factor: 16.971

4. Evolution of yeast ribosomal DNA: molecular cloning of the rDNA units of Kluyveromyces lactis and Hansenula wingei and their comparison with the rDNA units of other Saccharomycetoideae.

Authors: M P Verbeet; H van Heerikhuizen; J Klootwijk; R D Fontijn; R J Planta
Journal: Mol Gen Genet Date: 1984

5. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1983-07-01 Impact factor: 3.365

6. Cloning and analysis of the Kluyveromyces lactis TRP1 gene: a chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3.

Authors: M J Stark; J S Milner
Journal: Yeast Date: 1989 Jan-Feb Impact factor: 3.239

7. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors: D C Schwartz; C R Cantor
Journal: Cell Date: 1984-05 Impact factor: 41.582

8. Deletions and rearrangements in Kluyveromyces lactis mitochondrial DNA.

Authors: C M Hardy; C L Galeotti; G D Clark-Walker
Journal: Curr Genet Date: 1989-12 Impact factor: 3.886

9. Analysis of chromosomal DNA patterns of the genus Kluyveromyces.

Authors: F Sor; H Fukuhara
Journal: Yeast Date: 1989 Jan-Feb Impact factor: 3.239

10. Karyotyping of yeast strains of several genera by field inversion gel electrophoresis.

Authors: J R Johnston; C R Contopoulou; R K Mortimer
Journal: Yeast Date: 1988-09 Impact factor: 3.239

10 in total

11 in total

1. In vivo conformation of mitochondrial DNA in fungi and zoosporic moulds.

Authors: R Maleszka; G D Clark-Walker
Journal: Curr Genet Date: 1992-10 Impact factor: 3.886

2. Magnification of the rDNA cluster in Kluyveromyces lactis.

Authors: R Maleszka; G D Clark-Walker
Journal: Mol Gen Genet Date: 1990-09

3. Centromeric DNA of Kluyveromyces lactis.

Authors: J J Heus; B J Zonneveld; H Y Steensma; J A Van den Berg
Journal: Curr Genet Date: 1990-12 Impact factor: 3.886

4. Nuclear mutations in the petite-negative yeast Schizosaccharomyces pombe allow growth of cells lacking mitochondrial DNA.

Authors: P Haffter; T D Fox
Journal: Genetics Date: 1992-06 Impact factor: 4.562