Literature DB >> 26114028

Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration.

Vikas Trivedi1, Thai V Truong2, Le A Trinh3, Daniel B Holland3, Michael Liebling4, Scott E Fraser3.   

Abstract

We present an imaging and image reconstruction pipeline that captures the dynamic three-dimensional beating motion of the live embryonic zebrafish heart at subcellular resolution. Live, intact zebrafish embryos were imaged using 2-photon light sheet microscopy, which offers deep and fast imaging at 70 frames per second, and the individual optical sections were assembled into a full 4D reconstruction of the beating heart using an optimized retrospective image registration algorithm. This imaging and reconstruction platform permitted us to visualize protein expression patterns at endogenous concentrations in zebrafish gene trap lines.

Entities:  

Keywords:  (170.1420) Biology; (170.3010) Image reconstruction techniques; (180.0180) Microscopy; (190.0190) Nonlinear optics

Year:  2015        PMID： 26114028      PMCID： PMC4473743          DOI： 10.1364/BOE.6.002056

Source DB:  PubMed          Journal:  Biomed Opt Express        ISSN： 2156-7085            Impact factor:   3.732


  25 in total

1.  Intracardiac fluid forces are an essential epigenetic factor for embryonic cardiogenesis.

Authors:  Jay R Hove; Reinhard W Köster; Arian S Forouhar; Gabriel Acevedo-Bolton; Scott E Fraser; Morteza Gharib
Journal:  Nature       Date:  2003-01-09       Impact factor: 49.962

2.  The embryonic vertebrate heart tube is a dynamic suction pump.

Authors:  Arian S Forouhar; Michael Liebling; Anna Hickerson; Abbas Nasiraei-Moghaddam; Huai-Jen Tsai; Jay R Hove; Scott E Fraser; Mary E Dickinson; Morteza Gharib
Journal:  Science       Date:  2006-05-05       Impact factor: 47.728

3.  Deep and fast live imaging with two-photon scanned light-sheet microscopy.

Authors:  Thai V Truong; Willy Supatto; David S Koos; John M Choi; Scott E Fraser
Journal:  Nat Methods       Date:  2011-07-17       Impact factor: 28.547

4.  Intravital imaging of cardiac function at the single-cell level.

Authors:  Aaron D Aguirre; Claudio Vinegoni; Matt Sebas; Ralph Weissleder
Journal:  Proc Natl Acad Sci U S A       Date:  2014-07-22       Impact factor: 11.205

5.  High-resolution imaging of cardiomyocyte behavior reveals two distinct steps in ventricular trabeculation.

Authors:  David W Staudt; Jiandong Liu; Kurt S Thorn; Nico Stuurman; Michael Liebling; Didier Y R Stainier
Journal:  Development       Date:  2014-01-08       Impact factor: 6.868

6.  Multicolor two-photon light-sheet microscopy.

Authors:  Pierre Mahou; Julien Vermot; Emmanuel Beaurepaire; Willy Supatto
Journal:  Nat Methods       Date:  2014-06       Impact factor: 28.547

Review 7.  Zebrafish genetics and vertebrate heart formation.

Authors:  D Y Stainier
Journal:  Nat Rev Genet       Date:  2001-01       Impact factor: 53.242

8.  Conditional gene-trap mutagenesis in zebrafish.

Authors:  Lisette A Maddison; Mingyu Li; Wenbiao Chen
Journal:  Methods Mol Biol       Date:  2014

9.  Light-sheet microscopy in thick media using scanned Bessel beams and two-photon fluorescence excitation.

Authors:  Florian O Fahrbach; Vasily Gurchenkov; Kevin Alessandri; Pierre Nassoy; Alexander Rohrbach
Journal:  Opt Express       Date:  2013-06-03       Impact factor: 3.894

10.  A public gene trap resource for mouse functional genomics.

Authors:  William C Skarnes; Harald von Melchner; Wolfgang Wurst; Geoff Hicks; Alex S Nord; Tony Cox; Stephen G Young; Patricia Ruiz; Phil Soriano; Marc Tessier-Lavigne; Bruce R Conklin; William L Stanford; Janet Rossant
Journal:  Nat Genet       Date:  2004-06       Impact factor: 38.330
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  7 in total

1.  Cell-accurate optical mapping across the entire developing heart.

Authors:  Michael Weber; Nico Scherf; Alexander M Meyer; Daniela Panáková; Peter Kohl; Jan Huisken
Journal:  Elife       Date:  2017-12-29       Impact factor: 8.140

2.  Integrated single- and two-photon light sheet microscopy using accelerating beams.

Authors:  Peeter Piksarv; Dominik Marti; Tuan Le; Angelika Unterhuber; Lindsey H Forbes; Melissa R Andrews; Andreas Stingl; Wolfgang Drexler; Peter E Andersen; Kishan Dholakia
Journal:  Sci Rep       Date:  2017-05-03       Impact factor: 4.379

Review 3.  Computational Modeling of Blood Flow Hemodynamics for Biomechanical Investigation of Cardiac Development and Disease.

Authors:  Huseyin Enes Salman; Huseyin Cagatay Yalcin
Journal:  J Cardiovasc Dev Dis       Date:  2021-01-31

Review 4.  Following the Beat: Imaging the Valveless Pumping Function in the Early Embryonic Heart.

Authors:  Shang Wang; Irina V Larina
Journal:  J Cardiovasc Dev Dis       Date:  2022-08-15

5.  Line excitation array detection fluorescence microscopy at 0.8 million frames per second.

Authors:  Chris Martin; Tianqi Li; Evan Hegarty; Peisen Zhao; Sudip Mondal; Adela Ben-Yakar
Journal:  Nat Commun       Date:  2018-10-29       Impact factor: 14.919

6.  A versatile, multi-laser twin-microscope system for light-sheet imaging.

Authors:  Kevin Keomanee-Dizon; Scott E Fraser; Thai V Truong
Journal:  Rev Sci Instrum       Date:  2020-05-01       Impact factor: 1.523

7.  High-contrast, synchronous volumetric imaging with selective volume illumination microscopy.

Authors:  Thai V Truong; Daniel B Holland; Sara Madaan; Andrey Andreev; Kevin Keomanee-Dizon; Josh V Troll; Daniel E S Koo; Margaret J McFall-Ngai; Scott E Fraser
Journal:  Commun Biol       Date:  2020-02-14
  7 in total

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