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Literature DB >> 26102235

Labeling Cell Surface GPIs and GPI-Anchored Proteins through Metabolic Engineering with Artificial Inositol Derivatives.

Abstract

Glycosylphosphatidylinositol (GPI) anchoring of proteins to the cell surface is important for various biological processes, but GPI-anchored proteins are difficult to study. An effective strategy was developed for the metabolic engineering of cell-surface GPIs and GPI-anchored proteins by using inositol derivatives carrying an azido group. The azide-labeled GPIs and GPI-anchored proteins were then tagged with biotin on live cells through a click reaction, which allows further elaboration with streptavidin-conjugated dyes or other molecules. The strategy can be used to label GPI-anchored proteins with various tags for biological studies.

Entities: CellLine Chemical Disease Species

Keywords: GPI anchor; carbohydrates; glycolipids; inositol; metabolic engineering

Mesh：

Substances：

Year: 2015 PMID： 26102235 PMCID： PMC4536913 DOI： 10.1002/anie.201503814

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

41 in total

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10 in total

Review 1. Programming Cell-Cell Interactions through Non-genetic Membrane Engineering.

Authors: Clifford M Csizmar; Jacob R Petersburg; Carston R Wagner
Journal: Cell Chem Biol Date: 2018-06-14 Impact factor: 8.116

2. A Membrane-Anchored Short-Peptide Fusion Inhibitor Fully Protects Target Cells from Infections of Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus.

Authors: Xiaoran Tang; Hongliang Jin; Yue Chen; Li Li; Yuanmei Zhu; Huihui Chong; Yuxian He
Journal: J Virol Date: 2019-10-29 Impact factor: 5.103

3. Labeling of Phosphatidylinositol Lipid Products in Cells through Metabolic Engineering by Using a Clickable myo-Inositol Probe.

Authors: Tanei J Ricks; Chelsi D Cassilly; Adam J Carr; Daiane S Alves; Shahrina Alam; Kathrin Tscherch; Timothy W Yokley; Cameron E Workman; Jennifer L Morrell-Falvey; Francisco N Barrera; Todd B Reynolds; Michael D Best
Journal: Chembiochem Date: 2018-10-17 Impact factor: 3.164

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Authors: Jian Gao; Zhifang Zhou; Jiatong Guo; Zhongwu Guo
Journal: Chem Commun (Camb) Date: 2017-06-06 Impact factor: 6.222

5. Synthesis and evaluation of N^α,N^ε-diacetyl-l-lysine-inositol conjugates as cancer-selective probes for metabolic engineering of GPIs and GPI-anchored proteins.

Authors: Mohit Jaiswal; Sanyong Zhu; Wenjie Jiang; Zhongwu Guo
Journal: Org Biomol Chem Date: 2020-04-15 Impact factor: 3.876