Sai-Ming Lau1, Indra Vythilingam2, Jonathan Inbaraj Doss3, Shamala Devi Sekaran4, Tock H Chua5, Wan Yusof Wan Sulaiman2, Karuthan Chinna3, Yvonne Ai-Lian Lim2, Balan Venugopalan1. 1. State Vector Borne Disease Control Unit, Selangor State Health Department, Selangor, Malaysia. 2. Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. 3. Julius Centre, Department of Social and Preventive Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. 4. Department of Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. 5. Department of Pathobiology and Medical Diagnostics, Faculty of Medicine and Health Sciences, University of Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia.
Abstract
OBJECTIVE: To determine the effectiveness of using sticky traps and the NS1 dengue antigen kit for the surveillance of Aedes mosquitoes for dengue control. METHODS: Apartments were selected in a dengue-endemic area, and sticky traps were set to capture adult Aedes mosquitoes. NS1 dengue antigen kit was used to detect dengue antigen in mosquitoes, and positive mosquitoes were serotyped using real-time RT-PCR. RESULTS: The sticky traps were effective in capturing Aedes aegypti, and a minimum of three traps per floor was sufficient. Multiple serotypes were found in individual mosquitoes. CONCLUSION: The sticky trap and the NS1 dengue antigen test kit can be used as surveillance tool in dengue control programmes. This proactive method will be better suited for control programmes than current reactive methods.
OBJECTIVE: To determine the effectiveness of using sticky traps and the NS1 dengue antigen kit for the surveillance of Aedes mosquitoes for dengue control. METHODS: Apartments were selected in a dengue-endemic area, and sticky traps were set to capture adult Aedes mosquitoes. NS1 dengue antigen kit was used to detect dengue antigen in mosquitoes, and positive mosquitoes were serotyped using real-time RT-PCR. RESULTS: The sticky traps were effective in capturing Aedes aegypti, and a minimum of three traps per floor was sufficient. Multiple serotypes were found in individual mosquitoes. CONCLUSION: The sticky trap and the NS1 dengue antigen test kit can be used as surveillance tool in dengue control programmes. This proactive method will be better suited for control programmes than current reactive methods.
Authors: Sivaneswari Selvarajoo; Jonathan Wee Kent Liew; Tock H Chua; Wing Tan; Rafdzah Ahmad Zaki; Romano Ngui; Wan Yusoff Wan Sulaiman; Poo Soon Ong; Indra Vythilingam Journal: Sci Rep Date: 2022-01-12 Impact factor: 4.996
Authors: Kimberly Fornace; Benny Obrain Manin; Jason Matthiopoulos; Heather M Ferguson; Chris Drakeley; Kamruddin Ahmed; Koay Teng Khoon; Robert M Ewers; Sylvia Daim; Tock Hing Chua Journal: Wellcome Open Res Date: 2022-02-16
Authors: André S Leandro; Wagner A Chiba de Castro; Renata D Lopes; Robson M Delai; Daniel A M Villela; Rafael Maciel de-Freitas Journal: Emerg Infect Dis Date: 2022-04 Impact factor: 6.883