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Literature DB >> 26086697

In vitro gene expression within membrane-free coacervate protocells.

T-Y Dora Tang¹, Dirk van Swaay, Andrew deMello, J L Ross Anderson, Stephen Mann.

Abstract

Cell-free gene expression of a fluorescent protein (mCherry) is demonstrated within the molecularly crowded matrix of a polysaccharide/polypeptide coacervate.

Entities: Chemical

Mesh：

Substances：

Year: 2015 PMID： 26086697 DOI： 10.1039/c5cc04220h

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

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Cited

35 in total

1. Enzymatic degradation of liquid droplets of DNA is modulated near the phase boundary.

Authors: Omar A Saleh; Byoung-Jin Jeon; Tim Liedl
Journal: Proc Natl Acad Sci U S A Date: 2020-06-29 Impact factor: 11.205

2. Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells.

Authors: Emiliano Altamura; Francesco Milano; Roberto R Tangorra; Massimo Trotta; Omar Hassan Omar; Pasquale Stano; Fabio Mavelli
Journal: Proc Natl Acad Sci U S A Date: 2017-03-20 Impact factor: 11.205

3. Liquid-liquid phase separation in artificial cells.

Authors: Charles D Crowe; Christine D Keating
Journal: Interface Focus Date: 2018-08-17 Impact factor: 3.906

4. Light-powered CO₂ fixation in a chloroplast mimic with natural and synthetic parts.

Authors: Tarryn E Miller; Thomas Beneyton; Thomas Schwander; Christoph Diehl; Mathias Girault; Richard McLean; Tanguy Chotel; Peter Claus; Niña Socorro Cortina; Jean-Christophe Baret; Tobias J Erb
Journal: Science Date: 2020-05-08 Impact factor: 47.728

Review 9. Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology.

Authors: Yuval Elani
Journal: Biochem Soc Trans Date: 2016-06-15 Impact factor: 5.407

Review 10. Connecting primitive phase separation to biotechnology, synthetic biology, and engineering.

Authors: Tony Z Jia; Po-Hsiang Wang; Tatsuya Niwa; Irena Mamajanov
Journal: J Biosci Date: 2021 Impact factor: 1.826

In vitro gene expression within membrane-free coacervate protocells.

1. Enzymatic degradation of liquid droplets of DNA is modulated near the phase boundary.

2. Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells.

3. Liquid-liquid phase separation in artificial cells.

4. Light-powered CO₂ fixation in a chloroplast mimic with natural and synthetic parts.

5. Incorporation and Assembly of a Light-Emitting Enzymatic Reaction into Model Protein Condensates.

Review 6. The role of liquid-liquid phase separation in regulating enzyme activity.

7. Enhanced Ribozyme-Catalyzed Recombination and Oligonucleotide Assembly in Peptide-RNA Condensates.

8. Artificial Gel-Based Organelles for Spatial Organization of Cell-Free Gene Expression Reactions.

Review 9. Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology.

Review 10. Connecting primitive phase separation to biotechnology, synthetic biology, and engineering.