Literature DB >> 26072151

Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards.

Tahaniyat Lalani1, Michele D Tisdale2, Jason D Maguire3, Chansuda Wongsrichanalai4, Mark S Riddle5, David R Tribble6.   

Abstract

We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman(™) FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 10(2) to 10(5)CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  PCR; Stool card; Travelers' diarrhea

Mesh：

Year:  2015        PMID： 26072151      PMCID： PMC4532578          DOI： 10.1016/j.diagmicrobio.2015.05.011

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


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Authors:  Aisha Al Amri; Abiola C Senok; Abdulrahman Yusuf Ismaeel; Ali E Al-Mahmeed; Giuseppe A Botta
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9.  PCR-based assay using occult blood detection cards for detection of diarrheagenic Escherichia coli in specimens from U.S. travelers to Mexico with acute diarrhea.

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Authors:  Mark S Riddle; Bradley A Connor; Nicholas J Beeching; Herbert L DuPont; Davidson H Hamer; Phyllis Kozarsky; Michael Libman; Robert Steffen; David Taylor; David R Tribble; Jordi Vila; Philipp Zanger; Charles D Ericsson
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10.  Comparison of stool collection and storage on Whatman FTA Elute cards versus frozen stool for enteropathogen detection using the TaqMan Array Card PCR assay.

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