Eliette Schultz1, Marisa Haenni2, Laurent Mereghetti3, Eliane Siebor4, Catherine Neuwirth4, Jean-Yves Madec2, Axel Cloeckaert5, Benoît Doublet6. 1. INRA, UMR1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, France ANSES, Unité Antibiorésistance et Virulence Bactériennes, F- 69364 Lyon, France. 2. ANSES, Unité Antibiorésistance et Virulence Bactériennes, F- 69364 Lyon, France. 3. INRA, UMR1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, France CHU de Tours, Service de Bactériologie-Virologie, F37044 Tours, France. 4. Laboratory of Bacteriology, University Hospital of Dijon, Plateau technique de Biologie, BP 37013, F-21070 Dijon cedex, France. 5. INRA, UMR1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, France. 6. INRA, UMR1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000 Tours, France benoit.doublet@tours.inra.fr.
Abstract
OBJECTIVES: To characterize MDR genomic islands related to Salmonella genomic island 1 (SGI1) and Proteus genomic island 1 (PGI1) in Proteus mirabilis from human and animal sources in France in light of the previously reported cases. METHODS: A total of 52 and 46 P. mirabilis clinical strains from human and animal sources, respectively, were studied for the period 2010-13. MDR was assessed by antimicrobial susceptibility testing, PCR detection of SGI1 and PGI1 and PCR mapping of the MDR regions. The diversity of the SGI1/PGI1-positive P. mirabilis strains was assessed by PFGE. RESULTS: Twelve P. mirabilis strains (5 humans and 7 dogs) were found to harbour an MDR island related to SGI1 or PGI1. Among them, several SGI1 variants were identified in diverse P. mirabilis genetic backgrounds. The variant SGI1-V, which harbours the ESBL bla VEB-6 gene, was found in closely genetically related human and dog P. mirabilis strains. The recently described PGI1 element was also identified in human and dog strains. Finally, one strain harboured a novel SGI genomic island closely related to SGI1 and SGI2 without an insertion of the MDR region. CONCLUSION: This study reports for the first time, to our knowledge, SGI1-positive and PGI1-positive P. mirabilis strains from dogs in France. The genetic diversity of the strains suggests several independent horizontal acquisitions of these MDR elements. The potential transmission of SGI1/PGI1-positive P. mirabilis strains between animals and humans is of public health concern, notably with regard to the spread of ESBL and carbapenemase genes, i.e. bla VEB-6 and bla NDM-1.
OBJECTIVES: To characterize MDR genomic islands related to Salmonella genomic island 1 (SGI1) and Proteus genomic island 1 (PGI1) in Proteus mirabilis from human and animal sources in France in light of the previously reported cases. METHODS: A total of 52 and 46 P. mirabilis clinical strains from human and animal sources, respectively, were studied for the period 2010-13. MDR was assessed by antimicrobial susceptibility testing, PCR detection of SGI1 and PGI1 and PCR mapping of the MDR regions. The diversity of the SGI1/PGI1-positive P. mirabilis strains was assessed by PFGE. RESULTS: Twelve P. mirabilis strains (5 humans and 7 dogs) were found to harbour an MDR island related to SGI1 or PGI1. Among them, several SGI1 variants were identified in diverse P. mirabilis genetic backgrounds. The variant SGI1-V, which harbours the ESBL bla VEB-6 gene, was found in closely genetically related human and dogP. mirabilis strains. The recently described PGI1 element was also identified in human and dog strains. Finally, one strain harboured a novel SGI genomic island closely related to SGI1 and SGI2 without an insertion of the MDR region. CONCLUSION: This study reports for the first time, to our knowledge, SGI1-positive and PGI1-positive P. mirabilis strains from dogs in France. The genetic diversity of the strains suggests several independent horizontal acquisitions of these MDR elements. The potential transmission of SGI1/PGI1-positive P. mirabilis strains between animals and humans is of public health concern, notably with regard to the spread of ESBL and carbapenemase genes, i.e. bla VEB-6 and bla NDM-1.