Literature DB >> 26063640

An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier.

Jisu Lee1, Eun Kyung Song, Yoonji Bae, Junseon Min, Hyun-Woo Rhee, Tae Joo Park, Moonil Kim, Sebyung Kang.   

Abstract

A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.

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Year:  2015        PMID： 26063640     DOI： 10.1039/c5cc02409a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  6 in total

1.  Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells.

Authors:  Jeffrey D Martell; Thomas J Deerinck; Stephanie S Lam; Mark H Ellisman; Alice Y Ting
Journal:  Nat Protoc       Date:  2017-08-10       Impact factor: 13.491

Review 2.  Protein Chemical Labeling Using Biomimetic Radical Chemistry.

Authors:  Shinichi Sato; Hiroyuki Nakamura
Journal:  Molecules       Date:  2019-11-03       Impact factor: 4.411

3.  Mapping Interactions between Glycans and Glycan-Binding Proteins by Live Cell Proximity Tagging.

Authors:  Eugene Joeh; Abigail E Reeves; Christopher G Parker; Mia L Huang
Journal:  Curr Protoc       Date:  2021-04

4.  RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses.

Authors:  Oliver Baker; Ashish Gupta; Mandy Obst; Youming Zhang; Konstantinos Anastassiadis; Jun Fu; A Francis Stewart
Journal:  Sci Rep       Date:  2016-05-24       Impact factor: 4.379

5.  A Recombinant Secondary Antibody Mimic as a Target-specific Signal Amplifier and an Antibody Immobilizer in Immunoassays.

Authors:  Junseon Min; Eun Kyung Song; Hansol Kim; Kyoung Taek Kim; Tae Joo Park; Sebyung Kang
Journal:  Sci Rep       Date:  2016-04-11       Impact factor: 4.379

6.  Ultrastructure of light-activated axons following optogenetic stimulation to produce late-phase long-term potentiation.

Authors:  Masaaki Kuwajima; Olga I Ostrovskaya; Guan Cao; Seth A Weisberg; Kristen M Harris; Boris V Zemelman
Journal:  PLoS One       Date:  2020-01-15       Impact factor: 3.240
  6 in total

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